1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out

Microbial Cell Factories

Research

Wim K Soetaert¹  Marjan De Mey¹  Joeri Beauprez¹  Sofie L De Maeseneire¹  Fatma G Avci²  Veerle ET Maervoet³ 

[1] Department of Biochemical and Microbial Technology, Centre of Expertise - Industrial Biotechnology and Biocatalysis, Ghent University, Coupure links 653, B-9000, Ghent, Belgium;Department of Biochemical and Microbial Technology, Centre of Expertise - Industrial Biotechnology and Biocatalysis, Ghent University, Coupure links 653, B-9000, Ghent, Belgium;Bioengineering Department, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey;Department of Biochemical and Microbial Technology, Centre of Expertise - Industrial Biotechnology and Biocatalysis, Ghent University, Coupure links 653, B-9000, Ghent, Belgium;Department of Applied Bioscience Engineering, Laboratory of Biochemistry and Brewing, Ghent University, Valentin Vaerwyckweg 1, 9000, Ghent, Belgium;

关键词: Citrobacter werkmanii;    Glycerol dehydrogenase;    3-hydroxypropionaldehyde;    1,3-propanediol;    Yield;   

DOI  :  10.1186/1475-2859-13-70

 received in 2014-03-07, accepted in 2014-05-09,  发布年份 2014

来源: Springer

【 摘 要 】

Background1,3-propanediol (PDO) is a substantially industrial metabolite used in the polymer industry. Although several natural PDO production hosts exist, e.g. Klebsiella sp., Citrobacter sp. and Clostridium sp., the PDO yield on glycerol is insufficient for an economically viable bio-process. Enhancing this yield via strain improvement can be achieved by disconnecting the production and growth pathways. In the case of PDO formation, this approach results in a microorganism metabolizing glycerol strictly for PDO production, while catabolizing a co-substrate for growth and maintenance. We applied this strategy to improve the PDO production with Citrobacter werkmanii DSM17579.ResultsGenetic tools were developed and used to create Citrobacter werkmanii DSM17579 ∆dhaD in which dhaD, encoding for glycerol dehydrogenase, was deleted. Since this strain was unable to grow on glycerol anaerobically, both pathways were disconnected. The knock-out strain was perturbed with 13 different co-substrates for growth and maintenance. Glucose was the most promising, although a competition between NADH-consuming enzymes and 1,3-propanediol dehydrogenase emerged.ConclusionDue to the deletion of dhaD in Citrobacter werkmanii DSM17579, the PDO production and growth pathway were split. As a consequence, the PDO yield on glycerol was improved 1,5 times, strengthening the idea that Citrobacter werkmanii DSM17579 could become an industrially interesting host for PDO production.

【 授权许可】

CC BY   
© Maervoet et al.; licensee BioMed Central Ltd. 2014