In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts

Lipids in Health and Disease

Research

Yassine Ben Ali¹  Youssef Gargouri¹  Maher Kammoun¹  Sofiane Bezzine¹  Sonia Miladi²  Mohamed Damak² 

[1] Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, Tunisia;Laboratoire de Chimie des Substances Naturelles, Faculté des Sciences de Sfax, University of Sfax, Tunisia;

关键词: Antioxidant Activity;    DPPH;    Water Extract;    Emodin;    Phytochemical Screening;   

DOI  :  10.1186/1476-511X-10-30

 received in 2011-01-12, accepted in 2011-02-11,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundIn the present work we determined the total phenolic content of Aloe vera leaf skin (AVLS) extracts by using various solvents (hexane, chloroform-ethanol (1/1), ethyl acetate, butanol and water). We have also evaluated the antioxidant and the anti-PLA2 properties of these extracts by measuring their inhibition potency on the human pro-inflammatory phospholipase A2 (group IIA).ResultsThe water extract exhibits the highest inhibitory effect with an IC50 = 0.22 mg/ml and interestingly no effect was observed on the digestive phospholipase A2 (group IB) even at a concentration of 5 mg/ml. Antioxidant activities were also analyzed and the most active extracts were observed when using chloroform ethanol (1/1) and ethyl acetate (IC50 = 0.274 and 0.326 mg/ml, respectively). Analysis of the total phenolic content reveals that the water extract, with the best anti-PLA2 effect, was poor in phenolic molecules (2 mg GAE/g). This latter value has to be compared with the chloroform-ethanol and the ethyl acetate extracts (40 and 23.8 mg GAE/g, respectively), mostly responsible for the antioxidant activity.ConclusionA significant correlation was established between the total phenolic content and the antioxidant capacity but not with the anti PLA2 activity. Results from phytochemical screening suggest that the anti PLA2 molecules were probably catechin tannins compounds.

【 授权许可】

Unknown   
© Kammoun et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.