In vivo gene expression in a Staphylococcus aureus prosthetic joint infection characterized by RNA sequencing and metabolomics: a pilot study

BMC Microbiology

Research Article

Jeppe Lund Nielsen¹  Raluca Georgiana Maltesen¹  Per Halkjær Nielsen¹  Kåre Lehmann Nielsen¹  Lone Heimann Larsen²  Yijuan Xu³  Trine Rolighed Thomsen³  Henrik Carl Schønheyder⁴  Vang Quy Le⁵ 

[1] Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajersvej 7H, 9220, Aalborg, Denmark;Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajersvej 7H, 9220, Aalborg, Denmark;Department of Clinical Microbiology, Aalborg University Hospital, Aalborg, Denmark;Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajersvej 7H, 9220, Aalborg, Denmark;The Danish Technological Institute, Life Science Division, Aarhus, Denmark;Department of Clinical Microbiology, Aalborg University Hospital, Aalborg, Denmark;Department of Clinical Medicine, Aalborg University Hospital, Aalborg, Denmark;Section for Molecular Diagnostics, Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark;

关键词: Staphylococcus aureus;    Joint infection;    Prosthesis;    In vivo gene expression;    Virulence;    Metabolism;    Siderophore;    RNA-seq;    NMR;    Metabolomics;   

DOI  :  10.1186/s12866-016-0695-6

 received in 2015-11-10, accepted in 2016-04-26,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundStaphylococcus aureus gene expression has been sparsely studied in deep-sited infections in humans. Here, we characterized the staphylococcal transcriptome in vivo and the joint fluid metabolome in a prosthetic joint infection with an acute presentation using deep RNA sequencing and nuclear magnetic resonance spectroscopy, respectively. We compared our findings with the genome, transcriptome and metabolome of the S. aureus joint fluid isolate grown in vitro.ResultFrom the transcriptome analysis we found increased expression of siderophore synthesis genes and multiple known virulence genes. The regulatory pattern of catabolic pathway genes indicated that the bacterial infection was sustained on amino acids, glycans and nucleosides. Upregulation of fermentation genes and the presence of ethanol in joint fluid indicated severe oxygen limitation in vivo.ConclusionThis single case study highlights the capacity of combined transcriptome and metabolome analyses for elucidating the pathogenesis of prosthetic infections of major clinical importance.

【 授权许可】

CC BY   
© Xu et al. 2016