Plasmodium berghei bio-burden correlates with parasite lactate dehydrogenase: application to murine Plasmodium diagnostics

Malaria Journal

Methodology

Michael F. Good¹  Michael R. Batzloff¹  Danielle I. Stanisic¹  Sai Lata De¹  Fabian Rivera²  Christian Engwerda² 

[1] Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia;QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia;

关键词: Malaria;    pLDH;    Plasmodium;    Parasite bio-burden;   

DOI  :  10.1186/s12936-015-1027-2

 received in 2015-09-04, accepted in 2015-12-02,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe spectrum of techniques to detect malaria parasites in whole blood is limited to measuring parasites in circulation. One approach that is currently used to enumerate total parasite bio-burden involves the use of bio-luminescent parasites. As an alternative approach, this study describes the use of a commercial ELISA human parasite lactate dehydrogenase (pLDH) detection kit to estimate total parasite bio-burden in murine malaria models.MethodsThe cross reactivity of pLDH in a commercial human malaria pLDH diagnostic kit was established in different components of blood for different murine malaria models. The use of pLDH as a measure of parasite bio-burden was evaluated by examining pLDH in relation to peripheral blood parasitaemia as determined by microscopy and calculating total parasite bio-burden using a bio-luminescent Plasmodium berghei ANKA luciferase parasite.ResultsThe pLDH antigen was detected in all four murine Plasmodium species and in all components of Plasmodium-infected blood. A significant correlation (r = 0.6922, P value <0.0001) was observed between total parasite bio-burden, measured as log average radiance, and concentration of pLDH units.ConclusionsThis high throughput assay is a suitable measure of total parasite bio-burden in murine malaria infections. Unlike existing methods, it permits the estimation of both circulating and sequestered parasites, allowing a more accurate assessment of parasite bio-burden.

【 授权许可】

CC BY   
© De et al. 2015