Discovery of serum biomarkers of alcoholic fatty liver in a rodent model: C-reactive protein

Journal of Biomedical Science

Research

Chun-Chao Chang¹  Chia-Chi Wang²  Ai-Sheng Ho³  Fu-Der Mai⁴  Jungshan Chang⁵  Chun-Chia Cheng⁶  Shu-Lin Liu⁷  Ling-Yun Chen⁷  Shui-Cheng Lee⁸ 

[1] Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University Hospital, Taipei, Taiwan;Division of Gastroenterology, Buddhist Tzu Chi General Hospital, Taipei branch, Taiwan;Division of Gastroenterology, Cheng Hsin General Hospital, Taipei, Taiwan;Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan;Department of Biochemistry, School of Medicine, Taipei Medical University, Taipei, Taiwan;Biomedical Mass Imaging Research Center, Taipei Medical University, Taipei, Taiwan;Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan;Department of Biochemistry, School of Medicine, Taipei Medical University, Taipei, Taiwan;Research Center For Biomedical Implants and Microsurgery Devices, Taipei Medical University, Taipei, Taiwan;Neuroscience Research Center, Taipei Medical University Hospital, Taipei, Taiwan;Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan;Institute of Nuclear Energy Research, Atomic Energy Council, Taoyuan, Taiwan;Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan;Institute of Nuclear Energy Research, Atomic Energy Council, Taoyuan, Taiwan;

关键词: alcoholic fatty liver;    biomarker;    C - reactive protein;    haptoglobin;    two-dimensional differential gel electrophoresis;   

DOI  :  10.1186/1423-0127-18-52

 received in 2011-03-25, accepted in 2011-08-01,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundExcessive consumption of alcohol contributes to alcoholic liver disease. Fatty liver is the early stage of alcohol-related liver disease. The aim of this study was to search for specific serological biomarkers of alcoholic fatty liver (AFL) compared to healthy controls, non-alcoholic fatty liver (NAFL) and liver fibrosis in a rodent model.MethodsSerum samples derived from animals with AFL, NAFL, or liver fibrosis were characterized and compared using two-dimensional differential gel electrophoresis. A matrix-assisted laser desorption ionization-time of flight tandem mass spectrometer in conjunction with mascot software was used for protein identification. Subsequently, Western blotting and flexible multi-analyte profiling were used to measure the expressions of the putative biomarkers present in the serum of animals and clinical patients.ResultsEight differential putative biomarkers were identified, and the two most differentiated proteins, including upregulated C-reactive protein (CRP) and downregulated haptoglobin (Hp), were further investigated. Western blotting validated that CRP was dramatically higher in the serum of AFL compared to healthy controls and other animals with liver disease of NAFL or liver fibrosis (p < 0.05). Moreover, we found that CRP and Hp were both lower in liver fibrosis of TAA-induced rats and clinical hepatitis C virus-infected patients.ConclusionThe results suggest that increased levels of CRP are an early sign of AFL in rats. The abnormally elevated CRP induced by ethanol can be used as a biomarker to distinguish AFL from normal or otherwise diseased livers.

【 授权许可】

Unknown   
© Liu et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.