Recombinant ArtinM activates mast cells

BMC Immunology

Research Article

Maria Helena S. Goldman¹  Nerry Tatiana Cecilio²  Maria Cristina Roque-Barreira²  Maria Célia Jamur²  Constance Oliver²  Valéria Cintra Barbosa-Lorenzi³  Patricia Andressa de Almeida Buranello⁴  Maria Cristina Pranchevicius⁵  Gabriela Pereira-da-Silva⁶ 

[1] Department of Biology, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil;Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil;Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil;Present address: Department of Biochemistry, Weill Cornell Medical College of Cornell University, New York, NY, USA;Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil;Present address: Department of Biological Sciences, Universidade Federal do Triangulo Mineiro, Uberaba, MG, Brazil;Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil;Present address: Department of Genetics and Evolution, Universidade Federal de São Carlos, São Carlos, SP, Brazil;Department of Maternal-Infant Nursing and Public Health, Escola de Enfermagem de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil;

关键词: Mast cells;    rArtinM;    ArtinM;    Degranulation;    Lectin;   

DOI  :  10.1186/s12865-016-0161-0

 received in 2015-09-22, accepted in 2016-06-23,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMast cells are hematopoietically derived cells that play a role in inflammatory processes such as allergy, as well as in the immune response against pathogens by the selective and rapid release of preformed and lipid mediators, and the delayed release of cytokines. The native homotetrameric lectin ArtinM, a D-mannose binding lectin purified from Artocarpus heterophyllus seeds, is one of several lectins that are able to activate mast cells. Besides activating mast cells, ArtinM has been shown to affect several biological responses, including immunomodulation and acceleration of wound healing. Because of the potential pharmacological application of ArtinM, a recombinant ArtinM (rArtinM) was produced in Escherichia coli. The current study evaluated the ability of rArtinM to induce mast cell degranulation and activation.ResultsThe glycan binding specificity of rArtinM was similar to that of jArtinM. rArtinM, via its CRD, was able to degranulate, releasing β-hexosaminidase and TNF-α, and to promote morphological changes on the mast cell surface. Moreover, rArtinM induced the release of the newly-synthesized mediator, IL-4. rArtinM does not have a co-stimulatory effect on the FcεRI degranulation via. The IgE-dependent mast cell activation triggered by rArtinM seems to be dependent on NFkB activation.ConclusionsThe lectin rArtinM has the ability to activate and degranulate mast cells via their CRDs. The present study indicates that rArtinM is a suitable substitute for the native form, jArtinM, and that rArtinM may serve as an important and reliable pharmacological agent.

【 授权许可】

CC BY   
© The Author(s). 2016