Field transcriptome revealed critical developmental and physiological transitions involved in the expression of growth potential in japonicarice

BMC Plant Biology

Research Article

Makoto Kusaba¹  Kaori Kamatsuki²  Hiroshi Minami²  Nobukazu Namiki²  Hinako Takehisa³  Yoshiaki Nagamura³  Kazuhiko Sugimoto³  Hirohiko Hirochika³  Yutaka Sato³  Baltazar Antonio³  Ritsuko Motoyama³ 

[1] Graduate School of Science, Hiroshima University, Higashi-Hiroshima, 739-8526, Hiroshima, Japan;Mitsubishi Space Software Co. Ltd., Takezono 1-6-1, 305-0032, Tsukuba, Ibaraki, Japan;National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan;

关键词: Gene Ontology;    Leaf Blade;    Shoot Apical Meristem;    Leaf Sheath;    Transcriptome Change;   

DOI  :  10.1186/1471-2229-11-10

 received in 2010-09-29, accepted in 2011-01-12,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundPlant growth depends on synergistic interactions between internal and external signals, and yield potential of crops is a manifestation of how these complex factors interact, particularly at critical stages of development. As an initial step towards developing a systems-level understanding of the biological processes underlying the expression of overall agronomic potential in cereal crops, a high-resolution transcriptome analysis of rice was conducted throughout life cycle of rice grown under natural field conditions.ResultsA wide range of gene expression profiles based on 48 organs and tissues at various developmental stages identified 731 organ/tissue specific genes as well as 215 growth stage-specific expressed genes universally in leaf blade, leaf sheath, and root. Continuous transcriptome profiling of leaf from transplanting until harvesting further elucidated the growth-stage specificity of gene expression and uncovered two major drastic changes in the leaf transcriptional program. The first major change occurred before the panicle differentiation, accompanied by the expression of RFT1, a putative florigen gene in long day conditions, and the downregulation of the precursors of two microRNAs. This transcriptome change was also associated with physiological alterations including phosphate-homeostasis state as evident from the behavior of several key regulators such as miR399. The second major transcriptome change occurred just after flowering, and based on analysis of sterile mutant lines, we further revealed that the formation of strong sink, i.e., a developing grain, is not the major cause but is rather a promoter of this change.ConclusionsOur study provides not only the genetic basis for functional genomics in rice but also new insight into understanding the critical physiological processes involved in flowering and seed development, that could lead to novel strategies for optimizing crop productivity.

【 授权许可】

Unknown   
© Sato et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.