Aggravation of ovalbumin-induced murine asthma by co-exposure to desert-dust and organic chemicals: an animal model study

Environmental Health

Research

Takayuki Shibamoto¹  Takamichi Ichinose²  Seiichi Yoshida²  Yasuhiro Yoshida³  Keiichi Arashidani³  Yahao Ren⁴  Guifan Sun⁵  Miao He⁵  Masataka Nishikawa⁶  Hirohisa Takano⁷ 

[1] Department of Environmental Toxicology, University of California, 95616, Davis, CA, USA;Department of Health Sciences, Oita University of Nursing and Health Sciences, Oita, Japan;Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, 807-8555, Fukuoka, Japan;Department of Nutritional and Food Hygiene, College of Public Health, China Medical University, Shenyang, China;Environment and Chronic Non-communicable Disease Research Center, School of Public Health, College of Public Health, China Medical University, 11001, Shenyang, China;Environmental Chemistry Division, National Institute for Environmental Studies, 305-8506, Ibaraki, Japan;Environmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, 615-8530, Kyoto, Japan;

关键词: Tar;    Polycyclic aromatic hydrocarbons;    Asian sand dust;    Ovalbumin;    Lung eosinophilia;    Cytokine and chemokine;   

DOI  :  10.1186/1476-069X-13-83

 received in 2014-07-03, accepted in 2014-10-09,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe organic chemicals present in Asian sand dust (ASD) might contribute to the aggravation of lung eosinophila. Therefore, the aggravating effects of the Tar fraction from ASD on ovalbumin (OVA)-induced lung eosinophilia were investigated.MethodsThe Tar fraction was extracted from ASD collected from the atmosphere in Fukuoka, Japan. ASD collected from the Gobi desert was heated at 360°C to inactivate toxic organic substances (H-ASD). ICR mice were instilled intratracheally with 12 different test samples prepared with Tar (1 μg and 5 μg), H-ASD, and OVA in a normal saline solution containing 0.02% Tween 80. The lung pathology, cytological profiles in the bronchoalveolar lavage fluid (BALF), inflammatory cytokines/chemokines in BALF and OVA-specific immunoglobulin in serum were investigated.ResultsSeveral kinds of polycyclic aromatic hydrocarbons (PAHs) were detected in the Tar sample. H-ASD + Tar 5 μg induced slight neutrophilic lung inflammation. In the presence of OVA, Tar 5 μg increased the level of eosinophils slightly and induced trace levels of Th2 cytokines IL-5 and IL-13 in BALF. Also mild to moderate goblet cell proliferation and mild infiltration of eosinophils in the submucosa of airway were observed. These pathological changes caused by H-ASD + OVA were relatively small. However, in the presence of OVA and H-ASD, Tar, at as low a level as 1 μg, induced severe eosinophil infiltration and proliferation of goblet cells in the airways and significantly increased Th2 cytokines IL-5 and IL-13 in BALF. The mixture showed an adjuvant effect on OVA-specific IgG1 production.ConclusionsThese results indicate that H-ASD with even low levels of Tar exacerbates OVA-induced lung eosinophilia via increases of Th2-mediated cytokines. These results suggest that ASD-bound PAHs might contribute to the aggravation of lung eosinophila.

【 授权许可】

Unknown   
© Ren et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.