Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence

Reproductive Biology and Endocrinology

Methodology

Hisashi Hashimoto¹  Hayato Yokoi²  Tohru Suzuki²  Yohei Matsuda²  Yoshie Ito³ 

[1] Bioscience and Biotechnology Center, Nagoya University, 464-8601, Nagoya, Japan;Laboratory of Marine Life Science and Genetics, Graduate School of Agricultural Science, Tohoku University, 981-8555, Aoba-ku,Sendai, Japan;Laboratory of Marine Life Science and Genetics, Graduate School of Agricultural Science, Tohoku University, 981-8555, Aoba-ku,Sendai, Japan;Industrial Technology Institute, Miyagi Prefectural Government, 981-3206, Sendai, Japan;

关键词: Vitellogenic Oocyte;    Yolk Granule;    Follicular Stimulate Hormone;    FITC Fluorescence;    Fluorescence Stereomicroscope;   

DOI  :  10.1186/1477-7827-9-45

 received in 2010-11-26, accepted in 2011-04-11,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundLarge volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.MethodsEstrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish.ResultsIncorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally.ConclusionThis method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.

【 授权许可】

Unknown   
© Matsuda et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.