Serum HOTAIR as a novel diagnostic biomarker for esophageal squamous cell carcinoma

Molecular Cancer

Short Communication

Xueting Hu¹  Minghui Wang¹  Xiaotian He¹  Zehua Zheng¹  Wenjian Wang¹  Xiaofan Ma¹  Duoguang Wu¹ 

[1] Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department of Thoracic surgery, Sun yat-sen memorial hospital, Sun yat-sen University, NO.107 of Yanjiangxi Road, 510120, Guangzhou, China;

关键词: Esophageal squamous cell carcinoma;    Long noncoding RNA;    HOTAIR;    Biomarker;    Diagnosis;   

DOI  :  10.1186/s12943-017-0643-6

 received in 2016-11-02, accepted in 2017-03-27,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundEarly diagnosis of esophageal squamous cell carcinoma (ESCC) is an important issue to improve the prognosis. HOX transcript antisense RNA (HOTAIR), a long noncoding RNA (lncRNA) expressed from the HOXC locus, has been recently revealed as an oncogenic regulator in ESCC. This study aimed to investigate whether serum HOTAIR is involved in the diagnosis of ESCC.MethodsIn this study, we detected serum HOTAIR expression in 50 patients with ESCC (including 42 tumor resection and 8 without surgery) and 20 healthy volunteers to investigate the role of serum HOTAIR in ESCC using the quantitative real-time polymerase chain reaction (qRT-PCR) method.ResultsClinical data indicated that serum HOTAIR were correlated with TNM stage. The expression level of serum HOTAIR (0.189 ± 0.010) was significantly higher in ESCC patients compared with that of healthy controls (0.055 ± 0.008, P < 0.01). The ROC curve analysis yielded an area under the ROC curve (AUC) value of 0.793 (95% CI: 0.692 to 0.895, P < 0.01). Also, the serum HOTAIR expression level decreased obviously in postoperative samples (one month after the surgery) compared to preoperative specimens. Moreover, there was a significant correlation between serum HOTAIR expression and the expression of HOTAIR in ESCC tissue according to Pearson correlation analysis.ConclusionsOur study, for the first time, demonstrated that serum HOTAIR might serve as a potential biomarker for the diagnosis of ESCC.

【 授权许可】

CC BY   
© The Author(s). 2017