Recent contributions in the field of the recombinant expression of disulfide bonded proteins in bacteria

Microbial Cell Factories

Commentary

Ario de Marco¹ 

[1] Department of Environmental Sciences, University of Nova Gorica, PO Box301, Vipavska 13, SI-5000, Rozna Dolina (Nova Gorica), Slovenia;

关键词: Isomerase;    Periplasmic Space;    Recombinant Antibody;    Leader Peptide;    Magnetic Cell Sorting;   

DOI  :  10.1186/1475-2859-11-129

 received in 2012-09-13, accepted in 2012-09-13,  发布年份 2012

来源: Springer

【 摘 要 】

The production of heterologous disulfide bonded proteins in bacteria remains a biotechnological challenge. A rapid literature survey results in the identification of some interesting proposals, such as the option of producing functional proteins in the cytoplasm in the presence of sulfhydryl oxidases and isomerases. Furthermore, an ever-increasing number of applications refers to recombinant proteins displayed at the bacterial surface. Time will tell whether these developments will lead to universally accepted laboratory protocols.

【 授权许可】

CC BY   
© de Marco; licensee BioMed Central Ltd. 2012