A reduced genome decreases the host carrying capacity for foreign DNA

Microbial Cell Factories

Research

Yuya Akeno¹  Saburo Tsuru¹  Tetsuya Yomo²  Bei-Wen Ying³ 

[1] Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, 565-0871, Suita, Osaka, Japan;Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, 565-0871, Suita, Osaka, Japan;Graduate School of Frontier Biosciences, Osaka University, 565-0871, Suita, Osaka, Japan;Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST), 565-0871, Suita, Osaka, Japan;Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Ibaraki, Japan;

关键词: Reduced genome;    Growth rate;    Exogenous DNAs;    Replication;    Plasmid carrying capacity;    Expression;    Escherichia coli;   

DOI  :  10.1186/1475-2859-13-49

 received in 2014-02-06, accepted in 2014-03-24,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundHost-plasmid interactions have been discussed largely in terms of the influences of plasmids, whereas the contributions of variations in host genomes to host interactions with foreign DNA remain unclear. A strain with a so-called “clean genome” (i.e., MDS42) of reduced genome size has recently been generated from the wild-type strain MG1655, a commonly used host strain. A quantitative evaluation of the influence of plasmid burdens in these two Escherichia coli strains can not only provide an understanding of how a reduced genome responds to foreign DNA but also offer insights into the proper application of these strains.ResultsThe decreases in growth caused by the cost of carrying foreign DNA were similar for the wild-type and clean-genome strains. A negative correlation between the growth rate and the total amount of exogenous DNA was observed in both strains, but a better theoretical fit with a higher statistical significance was found for the strain with the clean genome. Compared to the wild-type strain, the clean-genome strain exhibited a reduced carrying capacity for exogenous DNA, which was largely attributed to its ability to restrict the replication of foreign DNA. A tendency to allocate energy and resources toward gene expression, but not DNA replication, was observed in the strain with the clean genome.ConclusionsThe possession of a clean genome constrained the plasmid copy number to a wild-type-equivalent load. The results indicate that the wild-type strain possesses a greater tolerance for foreign DNA, as in endosymbiosis, and that the use of strains with clean genomes will be favorable in the applications that require precise control and theoretical prediction.

【 授权许可】

Unknown   
© Akeno et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.