Effects of Kurozu concentrated liquid on adipocyte size in rats

Lipids in Health and Disease

Research

Kumi Nakamura¹  Yoshie Kondo¹  Katsumi Imaizumi²  Sayaka Kawamura²  Li-Tao Tong²  Sanae Baba²  Masao Sato²  Yasutake Tanaka²  Yoshinori Katakura³  Miyako Udono³ 

[1] EGAO CO., LTD, 1-47, Higashihon-machi, 862-0902, Kumamoto, Japan;Laboratory of Nutrition Chemistry, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-Ku, 812-8581, Fukuoka, Japan;Lavoratory of Cellular Regulation Technology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-Ku, 812-8581, Fukuoka, Japan;

关键词: Subcutaneous Adipose Tissue;    Adipocyte Size;    Mesenteric Adipose Tissue;    Perirenal Adipose Tissue;    Pancreatic Lipase Activity;   

DOI  :  10.1186/1476-511X-9-134

 received in 2010-11-03, accepted in 2010-11-23,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundKurozu concentrated liquid (KCL) is used as a health-promoting supplement for the treatment of disorders such as cancer, hyperlipidemia, and hypertension in Japan. We investigated the possible anti-obesity effects of KCL in rats.MethodsMale Sprague Dawley rats were fed American Institute of Nutrition 76 formula diet and were orally administrated KCL or acetic acid at a dose of 100 mg/kg body weight or deionized water for 4 weeks. Adipocyte size, DNA content in subcutaneous adipose tissue, lipid levels in the serum and liver, and the rate of fatty acid excretion were determined. Effects of KCL on pancreatic lipase activity and 3T3-L1 preadipocyte differentiation were investigated in vitro.ResultsIn the KCL group, the average adipocyte size in subcutaneous and perirenal adipose tissues was significantly reduced. The KCL-administered rats displayed greater numbers of small adipocytes in the subcutaneous, perirenal and mesenteric adipose tissues than did rats from the other groups. In the KCL group, the DNA content in subcutaneous adipose tissue was significantly increased. The rate of fatty acid excretion was significantly increased in the KCL group. Furthermore, KCL significantly inhibited pancreatic lipase activity in vitro, and also significantly inhibited fat accumulation and mRNA expression of fatty acid binding protein 2 (aP2) and peroxisome proliferator-activated γ (PPARγ) in 3T3-L1 preadipocyte. The levels of serum and liver lipids, the concentration of serum glucose, and the levels of adiponectin were similar among the 3 groups.ConclusionOral administration of KCL decreases the adipocyte size via inhibition of dietary fat absorption and reductions of PPARγ and aP2 mRNA expression levels in adipocytes.

【 授权许可】

Unknown   
© Tong et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.