Genome-wide discovery and validation of Eucalyptus small RNAs reveals variable patterns of conservation and diversity across species of Myrtaceae

BMC Genomics

Research Article

Marília de Castro Rodrigues Pappas¹  Dario Grattapaglia²  Georgios Joannis Pappas³ 

[1] Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil;Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil;Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF, Brazil;Universidade de Brasília, Brasília, DF, Brazil;

关键词: Deep sequencing;    Eucalyptus;    Genome mapping;    Interspecific variability;    micro RNAs;    Myrtaceae;    Small RNAs;   

DOI  :  10.1186/s12864-015-2322-6

 received in 2015-06-19, accepted in 2015-12-15,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundMicro RNAs are a class of small non coding RNAs of 20–24 nucleotides transcribed as single stranded precursors from MIR gene loci. Initially described as post-transcriptional regulators involved in development, two decades ago, miRNAs have been proven to regulate a wide range of processes in plants such as germination, morphology and responses to biotic and abiotic stress. Despite wide conservation in plants, a number of miRNAs are lineage specific. We describe the first genome wide survey of Eucalyptus miRNAs based on high throughput sequencing.ResultsIn addition to discovering small RNA sequences, MIR loci were mapped onto the reference genome and interspecific variability investigated. Sequencing was carried out for the two most world widely planted species, E. grandis and E. globulus. To maximize discovery, E. grandis samples were from BRASUZ1, the same tree whose genome provided the reference sequence. Interspecific analysis reinforces the variability in small RNA repertoire even between closely related species. Characterization of Eucalyptus small RNA sequences showed 95 orthologous to conserved miRNAs and 193 novel miRNAs. In silico target prediction confirmed 163 novel miRNAs and degradome sequencing experimentally confirmed several hundred targets. Experimental evidence based on the exclusive expression of a set of small RNAs across 16 species within Myrtaceae further highlighted variable patterns of conservation and diversity of these regulatory elements.ConclusionsThe description of miRNAs in Eucalyptus contributes to scientific knowledge of this vast genre, which is the most widely planted hardwood crop in the tropical and subtropical world, adding another important element to the annotation of Eucalyptus grandis reference genome.

【 授权许可】

CC BY   
© Pappas et al. 2015