Luminescence-based in vivo monitoring of NF-κB activity through a gene delivery approach

Cell Communication and Signaling

Methodology

Fernando G Osorio¹  Jorge de la Rosa¹  José MP Freije¹ 

[1] Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncología, Universidad de Oviedo, 33006, Oviedo, Spain;

关键词: Gene Delivery;    Luciferin;    Sodium Salicylate;    Bioluminescence Signal;    Heat Lamp;   

DOI  :  10.1186/1478-811X-11-19

 received in 2013-02-19, accepted in 2013-03-11,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundMonitoring activity of specific signaling pathways in vivo is challenging and requires highly sensitive methods to detect dynamic perturbations in whole organisms.ResultsIn vivo gene delivery of a luciferase reporter followed by bioluminiscence imaging allows measuring NF-κB activity in mice liver and lungs.ConclusionsThis protocol allows a direct measure of NF-κB activity through quantification of bioluminescence signal, demonstrating its accuracy and sensitivity in different animal models and experimental conditions. Variants could be also applied for the analysis of NF-κB activity in different tissues or for studying other signaling pathways in vivo.

【 授权许可】

Unknown   
© Osorio et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.