Proteomic analysis of anthers from wild-type and photosensitive genetic male sterile mutant cotton (Gossypium hirsutum L.)

BMC Plant Biology

Research Article

Shuxun Yu¹  Ji Liu¹  Yanyan Meng²  Hengling Wei³  Shuli Fan³  Meizhen Song³  Chaoyou Pang³ 

[1] College of Agronomy, Northwest A&F University, 712100, Yangling, Shaanxi Province, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, 455000, Anyang, Henan Province, China;Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Soiences, South Central University for Nationalities, 430064, Wuhan, Hubei Province, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, 455000, Anyang, Henan Province, China;

关键词: Pollen Tube;    Male Sterility;    Cytoplasmic Male Sterility;    Pollen Tube Growth;    Pollen Development;   

DOI  :  10.1186/s12870-014-0390-4

 received in 2014-07-15, accepted in 2014-12-17,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundMale sterility is a common phenomenon in flowering plant species, and it has been successfully developed in several crops by taking advantage of heterosis. Using space mutation breeding of upland cotton, a novel photosensitive genetic male sterile (PGMS) mutant was isolated. To take advantage of the PGMS lines in cotton hybrid breeding, it is of great importance to study the molecular mechanisms of its male sterility.ResultsDelayed degradation of the PGMS anther tapetum occurred at different developmental stages as shown by analysis of anther cross-sections. To gain detailed insights into the cellular defects that occurred during PGMS pollen development, we used a differential proteomic approach to investigate the protein profiles of mutant and wild-type anthers at the tetrad, uninucleate and binucleate pollen stages. This approach identified 62 differentially expressed protein spots, including 19 associated with energy and metabolic pathways, 7 involved with pollen tube growth, 5 involved with protein metabolism, and 4 involved with pollen wall development. The remaining 27 protein spots were classified into other functional processes, such as protein folding and assembly (5 spots), and stress defense (4 spots). These differentially expressed proteins strikingly affected pollen development in the PGMS mutant anther and resulted in abnormal pollen grain formation, which may be the key reason for its male sterility.ConclusionsThis work represents the first study using comparative proteomics between fertile and PGMS cotton plants to identify PGMS-related proteins. The results demonstrate the presence of a complicated metabolic network in anther development and advance our understanding of the molecular mechanisms of microgamete formation, providing insights into the molecular mechanisms of male sterility.

【 授权许可】

Unknown   
© Liu et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.