Fine-mapping and cross-validation of QTLs linked to fatty acid composition in multiple independent interspecific crosses of oil palm

BMC Genomics

Research Article

Johannes Jansen¹  Maizura Ithnin²  Zulkifli Yaakub²  Mohd Din Amiruddin²  Ravigadevi Sambanthamurthi²  Rajinder Singh²  Rozana Rosli²  Kuang-Lim Chan²  Katialisa Kamaruddin²  Rajanaidu Nookiah²  Leslie Eng Ti Low²  Ahmad Kushairi²  Nadzirah Amiruddin²  Ngoot-Chin Ting³  Sean Mayes⁴  Festo Massawe⁵  Xaviar Arulandoo⁶  Chin Ching Lim⁶  Kandha Sritharan⁶ 

[1] Biometris, Wageningen University and Research Centre, P.O. Box 100, 6700 AC, Wageningen, The Netherlands;Malaysian Palm Oil Board (MPOB), P.O. Box 10620, 50720, Kuala Lumpur, Malaysia;Malaysian Palm Oil Board (MPOB), P.O. Box 10620, 50720, Kuala Lumpur, Malaysia;School of Biosciences, University of Nottingham Malaysia Campus, Jalan Broga, 43500, Semenyih, Selangor, Malaysia;Plant and Crop Sciences, Sutton Bonington Campus, University of Nottingham, Sutton Bonington, LE12 5RD, Loughborough, UK;School of Biosciences, University of Nottingham Malaysia Campus, Jalan Broga, 43500, Semenyih, Selangor, Malaysia;United Plantations Bhd., Jendarata Estate, 36009, Teluk Intan, Perak, Malaysia;

关键词: Elaeis guineensis;    Elaeis oleifera;    Backcross-two (BC);    Iodine value (IV);    Palmitic acid (C16:0);    Oleic acid (C18:1);   

DOI  :  10.1186/s12864-016-2607-4

 received in 2015-08-22, accepted in 2016-03-28,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe commercial oil palm (Elaeis guineensis Jacq.) produces a mesocarp oil (commonly called ‘palm oil’) with approximately equal proportions of saturated and unsaturated fatty acids (FAs). An increase in unsaturated FAs content or iodine value (IV) as a measure of the degree of unsaturation would help to open up new markets for the oil. One way to manipulate the fatty acid composition (FAC) in palm oil is through introgression of favourable alleles from the American oil palm, E. oleifera, which has a more unsaturated oil.ResultsIn this study, a segregating E. oleifera x E. guineensis (OxG) hybrid population for FAC is used to identify quantitative trait loci (QTLs) linked to IV and various FAs. QTL analysis revealed 10 major and two putative QTLs for IV and six FAs, C14:0, C16:0, C16:1, C18:0, C18:1 and C18:2 distributed across six linkage groups (LGs), OT1, T2, T3, OT4, OT6 and T9. The major QTLs for IV and C16:0 on LGOT1 explained 60.0 – 69.0 % of the phenotypic trait variation and were validated in two independent BC2 populations. The genomic interval contains several key structural genes in the FA and oil biosynthesis pathways such as PATE/FATB, HIBCH, BASS2, LACS4 and DGAT1 and also a relevant transcription factor (TF), WRI1. The literature suggests that some of these genes can exhibit pleiotropic effects in the regulatory networks of these traits. Using the whole genome sequence data, markers tightly linked to the candidate genes were also developed. Clustering trait values according to the allelic forms of these candidate markers revealed significant differences in the IV and FAs of the palms in the mapping and validation crosses.ConclusionsThe candidate gene approach described and exploited here is useful to identify the potential causal genes linked to FAC and can be adopted for marker-assisted selection (MAS) in oil palm.

【 授权许可】

CC BY   
© Ting et al. 2016