High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

Microbial Cell Factories

Technical Notes

Antti Vasala¹  Kaisa Ukkonen²  Peter Neubauer³  Heikki Ojamo⁴ 

[1] BioSilta Oy, P. O. Box 4300, FI-90014, Oulu, Finland;BioSilta Oy, P. O. Box 4300, FI-90014, Oulu, Finland;Department of Process and Environmental Engineering, Bioprocess Engineering Laboratory, University of Oulu, P. O. Box 4300, FI-90014, Oulu, Finland;Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universität Berlin, D-13355, Ackerstrasse 71-76, Berlin, Germany;Department of Process and Environmental Engineering, Bioprocess Engineering Laboratory, University of Oulu, P. O. Box 4300, FI-90014, Oulu, Finland;

关键词: Shake flask;    Oxygen transfer;    Recombinant protein;    Fed-batch;   

DOI  :  10.1186/1475-2859-10-107

 received in 2011-10-05, accepted in 2011-12-12,  发布年份 2011

来源: Springer

【 摘 要 】

This report describes the combined use of an enzyme-based glucose release system (EnBase®) and high-aeration shake flask (Ultra Yield Flask™). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydrogenase expressed in E. coli. Compared to Terrific Broth and ZYM-5052 autoinduction medium, the EnBase system improved yield mainly through increased productivity per cell. Four-fold increase in oxygen transfer by the Ultra Yield Flask contributed to higher cell density with EnBase but not with the other tested media, and consequently the product yield per ml of EnBase culture was further improved.

【 授权许可】

CC BY   
© Ukkonen et al; licensee BioMed Central Ltd. 2011