Quantifying western blots: none more black

BMC Biology

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Graham Bell¹ 

[1] BMC Biology, London, UK;

关键词: Intensity Measurement;    Relative Quantification;    Quantification Analysis;    Linear Dynamic Range;    Western Blot Experiment;   

DOI  :  10.1186/s12915-016-0339-1

来源: Springer

【 摘 要 】

ndWestern blotting is among the most common techniques used in molecular biology and a simple way of assessing the presence or absence of a protein. It is also commonly used to compare protein levels in different conditions or in different tissues. This article illustrates some of the easy ways to arrive at a false conclusion when trying to quantify protein levels from western blots.

【 授权许可】

CC BY   
© The Author(s). 2016