Using ionic liquids in whole-cell biocatalysis for the nucleoside acylation

Microbial Cell Factories

Research

Furao Lai¹  Yan Lian¹  Xiaofeng Li¹  Hui Wu¹  Yao Ren²  Guanglei Zhao²  Meiyan Yang² 

[1] College of Light Industry and Food Sciences, South China University of Technology, 510641, Guangzhou, China;State Key lab of Pulp & Paper making Engineering, South China University of Technology, 510641, Guangzhou, China;College of Light Industry and Food Sciences, South China University of Technology, 510641, Guangzhou, China;

关键词: Biocatalysis;    Whole-cells;    Ionic liquid;    Nucleoside ester;    Regioselectivity;   

DOI  :  10.1186/s12934-014-0143-y

 received in 2014-05-26, accepted in 2014-09-23,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe use of biocatalysts has become an increasingly attractive alternative to traditional chemical methods, due to the high selectivity, mild reaction conditions and environmentally-friendly processes in nonaqueous catalysis of nucleosids. However, the extensive use of organic solvents may generally suffer from sever drawbacks such as volatileness and toxicity to the environment and lower activity of the biocatalyst. Recently, ionic liquids are considered promising solvents for nonaqueous biocatalysis of polyhydroxyl compounds as ILs are environmental-friendly.ResultsIn this research, we developed new IL-containing reaction systems for synthesis of long chain nucleoside ester catalyzed by Pseudomonas fluorescens whole-cells. Various ILs exerted significant but different effects on the bio-reaction. And their effects were closely related with both the anions and cations of the ILs. Use of 10% [BMI][PF6]/THF gave high reaction efficiency of arabinocytosine laurate synthesis, in which the initial rate, product yield and 5′-regioselectivity reached 2.34 mmol/L·h, 81.1% and >99%, respectively. Furthermore, SEM analysis revealed that ILs can alter the cell surface morphology, improve the permeability of cell envelopes and thus facilitate the mass transfer of substrates to the active sites of cell-bound enzymes.ConclusionOur research demonstrated the potential of ILs as promising reaction medium for achieving highly efficient and regioselective whole-cell catalysis.

【 授权许可】

Unknown   
© Yang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.