Mannan-binding lectin regulates dendritic cell maturation and cytokine production induced by lipopolysaccharide

BMC Immunology

Research Article

Zhengliang Chen¹  Yani Zhang¹  Xiao Lu¹  Yue Chen¹  Liyun Zhang¹  Mingyong Wang² 

[1] Department of Immunology, Southern Medical University, 510515, Guangzhou, PR, China;Department of Immunology, Southern Medical University, 510515, Guangzhou, PR, China;Department of Medical Tests, Xinxiang Medical University, 453003, Xinxiang, PR, China;

关键词: Electrophoretic Mobility Shift Assay;    Limulus Amebocyte Lysate Assay;    Collectin Family;    Physiological Calcium Concentration;    Immature MDCs;   

DOI  :  10.1186/1471-2172-12-1

 received in 2010-09-30, accepted in 2011-01-01,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundMannan-binding lectin (MBL) is a pattern-recognition molecule present in serum, which is involved in the innate immune defense by activating complement and promoting opsonophagocytosis. Dendritic cells (DCs) are professional antigen presenting cells (APCs) that are crucial for the initiation of adaptive immunity. Lipopolysaccharide (LPS) has been shown to be a strong activator of the inflammatory response and immune regulation. We first examined whether MBL modulated LPS-induced cellular responses, then investigated possible mechanisms of its inhibitory effect.ResultsMBL at higher concentrations (10-20 μg/ml) significantly attenuated LPS-induced maturation of monocyte-derived DCs (MDCs) and production of proinflammatory cytokines (e.g., IL-12 and TNF-α), and inhibited their ability to activate allogeneic T lymphocytes. It bound to immature MDCs at physiological calcium concentrations, and was optimal at supraphysiological calcium concentrations. MBL also bound directly to immature MDCs and attenuated the binding of LPS to the cell surfaces, resulting in decreased LPS-induced nuclear factor-κB (NF-κB) activity in these cells.ConclusionAll these data suggest that MBL could affect the functions of DCs by modifying LPS-induced cellular responses. This study supports an important role for MBL in the regulation of adaptive immune responses and inflammatory responses.

【 授权许可】

Unknown   
© Wang et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.