Spontaneous penetration of gold nanoparticles through the blood brain barrier (BBB)

Journal of Nanobiotechnology

Research

Paz Elia¹  Raya Zach¹  Hagit Sela²  Yehuda Zeiri²  Zeev Karpas³  Hagit Cohen⁴ 

[1] Department of Biomedical Engineering, Ben-Gurion University of the Negev, 8410501, Beer-Sheva, Israel;Department of Biomedical Engineering, Ben-Gurion University of the Negev, 8410501, Beer-Sheva, Israel;Department of Chemistry, NRCN, P.O. Box 9001, 8419001, Beer-Sheva, Israel;Department of Chemistry, NRCN, P.O. Box 9001, 8419001, Beer-Sheva, Israel;The State of Israel Ministry of Health, Anxiety and Stress Research Unit, Faculty of Health Sciences, Beer-Sheva Mental Health Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel;

关键词: Gold nanoparticles;    Rat;    Biokinetics;    Penetration;    Blood-brain barrier;    ICP-MS;    LA-ICP-MS;   

DOI  :  10.1186/s12951-015-0133-1

 received in 2015-06-27, accepted in 2015-10-06,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe blood brain barrier (BBB) controls the brain microenvironment and limits penetration of the central nervous system (CNS) by chemicals, thus creating an obstacle to many medical imaging and treatment procedures. Research efforts to identify viable routes of BBB penetration have focused on structures such as micelles, polymeric nanoparticles and liposomes as drug carriers, however, many of them failed to provide unequivocal proof of BBB penetration. Here we proved that gold nanoparticles (AuNPs) penetrate the BBB in rats to reach brain regions.ResultsInjection of AuNPs to the abdominal cavity of rats resulted in levels of gold found in blood, urine, brain regions and body organs. After perfusion the concentration of gold in brain regions diminished dramatically indicating that most of the gold was in venous blood and not in the brain tissues. Injection of Na, K or Ca ion channel blockers reduced BBB penetration by half. A biological half-life of 12.9 ± 4.9 h was found for the gold nanoparticles. Possible mechanisms for the transport of AuNPs through the BBB are discussed.ConclusionsBBB penetration by AuNPs is spontaneous without the application of an external field. A major amount of gold resides in blood vessels therefore perfusion required. Ion channel blockers can be used to control the transport of AuNPs.

【 授权许可】

CC BY   
© Sela et al. 2015