The anaerobic linalool metabolism in Thauera linaloolentis 47 Lol

BMC Microbiology

Research Article

Robert Marmulla¹  Edinson Puentes Cala¹  Jens Harder¹  Thomas Schweder²  Stephanie Markert² 

[1] Department of Microbiology, Max Planck Institute for Marine Microbiology, Celsiusstr. 1, D-28359, Bremen, Germany;Department of Pharmaceutical Biotechnology, Institute for Pharmacy, University of Greifswald, Felix-Hausdorff-Str. 3, D-17487, Greifswald, Germany;

关键词: Monoterpene;    Linalool;    Geraniol;    Acyclic terpene utilization;   

DOI  :  10.1186/s12866-016-0693-8

 received in 2015-11-09, accepted in 2016-04-14,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe betaproteobacterium Thauera linaloolentis 47LolT was isolated on the tertiary monoterpene alcohol (R,S)-linalool as sole carbon and energy source under denitrifying conditions. Growth experiments indicated the formation of geraniol and geranial. Thus, a 3,1-hydroxyl-Δ1-Δ2-mutase (linalool isomerase) activity may initiate the degradation, followed by enzymes of the acyclic terpene utilization (Atu) and leucine/isovalerate utilization (Liu) pathways that were extensively studied in Pseudomonas spp. growing on citronellol or geraniol.ResultsA transposon mutagenesis yielded 39 transconjugants that could not grow anaerobically on linalool and nitrate in liquid medium. The deficiencies were apparently based on gene functions required to overcome the toxicity of linalool, but not due to inactivation of genes in the degradation pathway. Growing cultures formed geraniol and geranial transiently, but also geranic acid. Analysis of expressed proteins detected several enzymes of the Atu and Liu pathways. The draft genome of T. linaloolentis 47LolT had atu and liu genes with homology to those of Pseudomonas spp..ConclusionThe in comparison to monoterpenes larger toxicity of monoterpene alcohols is defeated by several modifications of the cellular structure and metabolism in Thauera linaloolentis 47LolT. The acyclic terpene utilization pathway is used in T. linaloolentis 47LolT during growth on (R,S)-linalool and nitrate under anoxic conditions. This is the first experimental verification of an active Atu pathway outside of the genus Pseudomonas.

【 授权许可】

CC BY   
© Marmulla et al. 2016