Changes in cardiac heparan sulfate proteoglycan expression and streptozotocin-induced diastolic dysfunction in rats

Cardiovascular Diabetology

Original Investigation

Célia MC Strunz¹  Antonio P Mansur¹  Ismar N Cestari¹  Adriana Nogueira¹  Vera MC Salemi¹  Monique Matsuda²  Monica V Marquezini³ 

[1] Heart Institute (InCor), University of São Paulo Medical School, 05403-000, São Paulo, Brazil;Laboratory of Investigation in Ophthalmology, University of São Paulo Medical School, 01246-903, São Paulo, Brazil;Pro-Sangue Foundation and Laboratory of Experimental Air Pollution of Pathology Department, University of São Paulo Medical School, 01246-903, São Paulo, Brazil;

关键词: Glypican;    Syndecan-4;    Diabetes;    Cardiac Muscle;   

DOI  :  10.1186/1475-2840-10-35

 received in 2011-02-22, accepted in 2011-04-25,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundChanges in the proteoglycans glypican and syndecan-4 have been reported in several pathological conditions, but little is known about their expression in the heart during diabetes. The aim of this study was to investigate in vivo heart function changes and alterations in mRNA expression and protein levels of glypican-1 and syndecan-4 in cardiac and skeletal muscles during streptozotocin (STZ)-induced diabetes.MethodsDiabetes was induced in male Wistar rats by STZ administration. The rats were assigned to one of the following groups: control (sham injection), after 24 hours, 10 days, or 30 days of STZ administration. Echocardiography was performed in the control and STZ 10-day groups. Western and Northern blots were used to quantify protein and mRNA levels in all groups. Immunohistochemistry was performed in the control and 30-day groups to correlate the observed mRNA changes to the protein expression.ResultsIn vivo cardiac functional analysis performed using echocardiography in the 10-day group showed diastolic dysfunction with alterations in the peak velocity of early (E) diastolic filling and isovolumic relaxation time (IVRT) indices. These functional alterations observed in the STZ 10-day group correlated with the concomitant increase in syndecan-4 and glypican-1 protein expression. Cardiac glypican-1 mRNA and skeletal syndecan-4 mRNA and protein levels increased in the STZ 30-day group. On the other hand, the amount of glypican in skeletal muscle was lower than that in the control group. The same results were obtained from immunohistochemistry analysis.ConclusionOur data suggest that membrane proteoglycans participate in the sequence of events triggered by diabetes and inflicted on cardiac and skeletal muscles.

【 授权许可】

CC BY   
© Strunz et al; licensee BioMed Central Ltd. 2011