An improved, low-cost, hydroponic system for growing Arabidopsis and other plant species under aseptic conditions

BMC Plant Biology

Methodology Article

Dolores Gutiérrez-Alanís¹  Betsy-Anaid Peña-Ocaña¹  Claudia-Anahí Pérez-Torres¹  Luis Herrera-Estrella¹  Lenin Yong-Villalobos¹  Sandra-Isabel González-Morales¹  Araceli Oropeza-Aburto¹  Fulgencio Alatorre-Cobos²  Carlos Calderón-Vázquez³  Alejandra Chacón-López⁴  Alfonso Méndez-Bravo⁵  Enrique Ibarra-Laclette⁵ 

[1] Laboratorio Nacional de Genómica para la Biodiversidad (Langebio)/Unidad de Genómica Avanzada (UGA), Centro de Investigación y Estudios Avanzados del IPN, 36500, Irapuato, Guanajuato, México;Laboratorio Nacional de Genómica para la Biodiversidad (Langebio)/Unidad de Genómica Avanzada (UGA), Centro de Investigación y Estudios Avanzados del IPN, 36500, Irapuato, Guanajuato, México;Department of Biological and Environmental Sciences, Institute of Biotechnology, University of Helsinki, 00014, Helsinki, Finland;Laboratorio Nacional de Genómica para la Biodiversidad (Langebio)/Unidad de Genómica Avanzada (UGA), Centro de Investigación y Estudios Avanzados del IPN, 36500, Irapuato, Guanajuato, México;Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Sinaloa, 81101, Guasave, Sinaloa, México;Laboratorio Nacional de Genómica para la Biodiversidad (Langebio)/Unidad de Genómica Avanzada (UGA), Centro de Investigación y Estudios Avanzados del IPN, 36500, Irapuato, Guanajuato, México;Instituto Tecnológico de Tepic, Laboratorio de Investigación Integral en Alimentos, División de Estudios de Posgrado, 63175, Tepic, Nayarit, México;Laboratorio Nacional de Genómica para la Biodiversidad (Langebio)/Unidad de Genómica Avanzada (UGA), Centro de Investigación y Estudios Avanzados del IPN, 36500, Irapuato, Guanajuato, México;Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C. Carretera Antigua a Coatepec #351, 91070, Xalapa, Veracruz, México;

关键词: Hydroponics;    Arabidopsis;    Root;    Phosphate starvation;    Pathogenesis;   

DOI  :  10.1186/1471-2229-14-69

 received in 2013-12-15, accepted in 2014-03-13,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundHydroponics is a plant growth system that provides a more precise control of growth media composition. Several hydroponic systems have been reported for Arabidopsis and other model plants. The ease of system set up, cost of the growth system and flexibility to characterize and harvest plant material are features continually improved in new hydroponic system reported.ResultsWe developed a hydroponic culture system for Arabidopsis and other model plants. This low cost, proficient, and novel system is based on recyclable and sterilizable plastic containers, which are readily available from local suppliers. Our system allows a large-scale manipulation of seedlings. It adapts to different growing treatments and has an extended growth window until adult plants are established. The novel seed-holder also facilitates the transfer and harvest of seedlings. Here we report the use of our hydroponic system to analyze transcriptomic responses of Arabidopsis to nutriment availability and plant/pathogen interactions.ConclusionsThe efficiency and functionality of our proposed hydroponic system is demonstrated in nutrient deficiency and pathogenesis experiments. Hydroponically grown Arabidopsis seedlings under long-time inorganic phosphate (Pi) deficiency showed typical changes in root architecture and high expression of marker genes involved in signaling and Pi recycling. Genome-wide transcriptional analysis of gene expression of Arabidopsis roots depleted of Pi by short time periods indicates that genes related to general stress are up-regulated before those specific to Pi signaling and metabolism. Our hydroponic system also proved useful for conducting pathogenesis essays, revealing early transcriptional activation of pathogenesis-related genes.

【 授权许可】

Unknown   
© Alatorre-Cobos et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.