Establishment and characterization of a metastasis model of human gastric cancer in nude mice

BMC Cancer

Research Article

Chunya Wang¹  Dandan Chai¹  Huifen Du¹  Kesheng Li¹  Xiaowen Lian¹  Xinwen Li²  Rong Yang³ 

[1]Department of Medicine Biotechnology, Medicine and Science Research Institute of Gansu province, Lanzhou, China

[2]Department of Surgery, Tumor Hospital of Gansu province, Lanzhou, China

[3]Department of pathology, Tumor Hospital of Gansu province, Lanzhou, China

关键词: Characterization;    Establishment;    Gastric cancer;    Metastasis;    Mouse models;   

DOI  :  10.1186/s12885-016-2101-z

 received in 2015-04-01, accepted in 2016-01-28,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundA mouse model of metastasis of human gastric cancer is one of the most important tools for studying the biological mechanisms underlying human gastric cancer metastasis. In this paper, we established a mouse model of metastatic human gastric cancer in nude mice that has a higher rate of tumor formation and metastasis than existing models.MethodsTo generate the mouse model of metastatic human gastric cancer, fresh tumor tissues from patients that have undergone surgery for gastric cancer were subcutaneously implanted in the right and left groins of nude mice. When the implanted tissue grew to 1 cubic centimeter, the mice were killed, and the tumor tissues were examined and resected. The tumor tissues were implanted into nude mice and subjected to pathological examination, immunohistochemical staining, and real-time PCR for cytokeratin 8/18 (CK8/18), E-cadherin, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). The mice were also analyzed for metastasis in their peritoneum, abdominal cavity, and internal organs by histopathological examination. Tissues collected from these organs were examined for pathology.ResultsAfter ten generations of implantation, all mice developed tumor growth at the implanted position, 94 % of the mice developed metastasis to the retroperitoneum and viscera. The implanted and metastatic tumor maintained the same histological features across all generations, and metastasis was observed in the esophagus, stomach, spleen, liver, kidney, adrenal, intestine, and pancreas. These metastatic tumors revealed no detectable expression of CK8/18, E-cadherin, VCAM-1, and ICAM-1.ConclusionsThis model will serve as valuable tool for understanding the metastatic process of human gastric cancer.

【 授权许可】

CC BY   
© Li et al. 2016