Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol

Microbial Cell Factories

Research

Klaas J. Hellingwerf¹  Aniek D. van der Woude¹  Ruth Perez Gallego¹  Vinod Puthan Veetil¹  Angie Vreugdenhil¹  Tania Chroumpi¹ 

[1] Photanol BV, Science Park 408, 1098XH, Amsterdam, The Netherlands;

关键词: Cyanobacteria;    Metabolic engineering;    Sweetener;    Erythritol;    Calvin cycle;    Erythrose;    Erythrose-4-phosphate;   

DOI  :  10.1186/s12934-016-0458-y

 received in 2016-01-18, accepted in 2016-03-28,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundErythritol is a polyol that is used in the food and beverage industry. Due to its non-caloric and non-cariogenic properties, the popularity of this sweetener is increasing. Large scale production of erythritol is currently based on conversion of glucose by selected fungi. In this study, we describe a biotechnological process to produce erythritol from light and CO2, using engineered Synechocystis sp. PCC6803.MethodsBy functionally expressing codon-optimized genes encoding the erythrose-4-phosphate phosphatase TM1254 and the erythrose reductase Gcy1p, or GLD1, this cyanobacterium can directly convert the Calvin cycle intermediate erythrose-4-phosphate into erythritol via a two-step process and release the polyol sugar in the extracellular medium. Further modifications targeted enzyme expression and pathway intermediates.ConclusionsAfter several optimization steps, the best strain, SEP024, produced up to 2.1 mM (256 mg/l) erythritol, excreted in the medium.

【 授权许可】

CC BY   
© van der Woude et al. 2016