Hemodynamic effects of intraoperative anesthetics administration in photothrombotic stroke model: a study using laser speckle imaging

BMC Neuroscience

Research Article

Lu Yuan¹  Hangdao Li¹  Xiaodan Lu¹  Bin Bo²  Hongyang Lu³  Yao Li³  Shanbao Tong³ 

[1] Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, 200030, Shanghai, China;School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, China;School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, China;Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, 200030, Shanghai, China;

关键词: Laser speckle imaging;    Photothrombotic stroke model;    Hemodynamic effect;    Anesthetics;   

DOI  :  10.1186/s12868-016-0327-y

 received in 2016-06-07, accepted in 2016-12-24,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundPrevious neuroimaging studies have shown the hemodynamic effect of either preconditioning or postconditioning anesthesia in ischemic stroke model. However, the anesthetic effect in hemodynamics during and immediately after the stroke modeling surgery remains unknown due to the lack of appropriate anesthesia-free stroke model and intraoperative imaging technology. In the present study, we utilized our recently developed photothrombotic model of focal cerebral ischemia in conscious and freely moving rats, and investigated transient hemodynamic changes with or without isoflurane administration. Laser speckle imaging was applied to acquire real-time two-dimensional full-field cerebral blood flow (CBF) information throughout the surgical operations and early after.ResultsSignificantly larger CBF reduction area was observed in conscious rats from 8 min immediately after the onset of stroke modeling, compared with anesthetized rats. Stroke rats without isoflurane administration also showed larger lesion volume identified by magnetic resonance imaging 3 h post occlusion (58.9%), higher neurological severity score 24 h post occlusion (28.3%), and larger infarct volume from 2,3,5-triphenyltetrazolium chloride staining 24 h post occlusion (46.9%).ConclusionsOur results demonstrated that the hemodynamic features were affected by anesthetics at as early as during the stroke induction. Also, our findings about the neuroprotection of intraoperative anesthetics administration bring additional insights into understanding the translational difficulty in stroke research.

【 授权许可】

CC BY   
© The Author(s) 2017