Vitamin D receptor regulates intestinal proteins involved in cell proliferation, migration and stress response

Lipids in Health and Disease

Research

Susann Weinholz¹  Christina Cordes¹  Angelika Schierhorn²  Hagen Kühne³  Alexandra Schutkowski³  Gabriele I Stangl³  Bettina König³  Kristin Schulz⁴ 

[1]Department of Applied Biosciences and Process Engineering, Anhalt University of Applied Sciences, D-06366, Köthen, Germany

[2]Institute for Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, D-06120, Halle (Saale), Germany

[3]Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Von-Danckelmann-Platz 2, D-06120, Halle (Saale), Germany

[4]Institute of Medical Immunology, Martin Luther University Halle-Wittenberg, D-06112, Halle (Saale), Germany

关键词: VDR-deficiency;    Small intestine;    Proteomics;    Laminin receptor;    Cell adhesion;    Mice;    Stress response;   

DOI  :  10.1186/1476-511X-13-51

 received in 2014-01-10, accepted in 2014-03-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundGenome-wide association studies found low plasma levels of 25-hydroxyvitamin D and vitamin D receptor (VDR) polymorphisms associated with a higher prevalence of pathological changes in the intestine such as chronic inflammatory bowel diseases.MethodsIn this study, a proteomic approach was applied to understand the overall physiological importance of vitamin D in the small intestine, beyond its function in calcium and phosphate absorption.ResultsIn total, 569 protein spots could be detected by two-dimensional-difference in-gel electrophoresis (2D-DIGE), and 82 proteins were considered as differentially regulated in the intestinal mucosa of VDR-deficient mice compared to that of wildtype (WT) mice. Fourteen clearly detectable proteins were identified by MS/MS and further analyzed by western blot and/or real-time RT-PCR. The differentially expressed proteins are functionally involved in cell proliferation, cell adhesion and cell migration, stress response and lipid transport. Mice lacking VDR revealed higher levels of intestinal proteins associated with proliferation and migration such as the 37/67 kDa laminin receptor, collagen type VI (alpha 1 chain), keratin-19, tropomyosin-3, adseverin and higher levels of proteins involved in protein trafficking and stress response than WT mice. In contrast, proteins that are involved in transport of bile and fatty acids were down-regulated in small intestine of mice lacking VDR compared to WT mice. However, plasma and liver concentrations of cholesterol and triglycerides were not different between the two groups of mice.ConclusionCollectively, these data imply VDR as an important factor for controlling cell proliferation, migration and stress response in the small intestine.

【 授权许可】

Unknown   
© Kühne et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.