Prospecting major genes in dairy buffaloes

BMC Genomics

Research Article

A. Reverter¹  R. Porto-Neto¹  SA Lehnert¹  SS Moore²  MRS Fortes³  DJA Santos⁴  RR Aspilcueta-Borquis⁴  GMF de Camargo⁴  H. Tonhati⁴  DF Cardoso⁴ 

[1] Commonwealth Scientific and Industrial Research Organization, Agriculture Flagship, St Lucia, 4072, Brisbane, QLD, Australia;Queensland Alliance for Agriculture and Food Innovation, Centre for Animal Science, The University of Queensland, 4067, Brisbane, QLD, Australia;School of Chemistry and Molecular Bioscience, The University of Queensland, St Lucia, 4072, Brisbane, QLD, Australia;Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Departamento de Zootecnia, Via de acesso Professor Paulo Donato Castelane, 14884-900, Jaboticabal, SP, Brazil;

关键词: Bubalus bubalis;    SNP;    Reproduction;    Milk;    Candidate genes;    GWAS;   

DOI  :  10.1186/s12864-015-1986-2

 received in 2015-03-11, accepted in 2015-10-06,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAsian buffaloes (Bubalus bubalis) have an important socio-economic role. The majority of the population is situated in developing countries. Due to the scarce resources in these countries, very few species-specific biotechnology tools exist and a lot of cattle-derived technologies are applied to buffaloes. However, the application of cattle genomic tools to buffaloes is not straightforward and, as results suggested, despite genome sequences similarity the genetic polymorphisms are different.ResultsThe first SNP chip genotyping platform designed specifically for buffaloes has recently become available. Herein, a genome-wide association study (GWAS) and gene network analysis carried out in buffaloes is presented. Target phenotypes were six milk production and four reproductive traits. GWAS identified SNP with significant associations and suggested candidate genes that were specific to each trait and also genes with pleiotropic effect, associated to multiple traits.ConclusionsNetwork predictions of interactions between these candidate genes may guide further molecular analyses in search of disruptive mutations, help select genes for functional experiments and evidence metabolism differences in comparison to cattle. The cattle SNP chip does not offer an optimal coverage of buffalo genome, thereafter the development of new buffalo-specific genetic technologies is warranted. An annotated reference genome would greatly facilitate genetic research, with potential impact to buffalo-based dairy production.

【 授权许可】

CC BY   
© de Camargo et al. 2015