Ectopic expression of AtPAD4broadens resistance of soybean to soybean cyst and root-knot nematodes

BMC Plant Biology

Research Article

Kyung-Hwan Kim¹  Eric P Brewer²  Gary R Bauchan²  Benjamin F Matthews²  Margaret H MacDonald²  Reham M Youssef³ 

[1] Cell and Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, 441-100, Suwon, South Korea;USDA-ARS, Soybean Genomic and Improvement Laboratory, 10300 Baltimore Ave, Bldg 006, 20705, Beltsville, MD, USA;USDA-ARS, Soybean Genomic and Improvement Laboratory, 10300 Baltimore Ave, Bldg 006, 20705, Beltsville, MD, USA;Plant Protection Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt;

关键词: Arabidopsis;    Phytoalexin-deficient4;    AtPAD4;    Soybean;    Nematodes;    Salicylic acid;    Plant defense;   

DOI  :  10.1186/1471-2229-13-67

 received in 2013-03-21, accepted in 2013-04-22,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe gene encoding PAD4 (PHYTOALEXIN-DEFICIENT4) is required in Arabidopsis for expression of several genes involved in the defense response to Pseudomonas syringae pv. maculicola. AtPAD4 (Arabidopsis thaliana PAD4) encodes a lipase-like protein that plays a regulatory role mediating salicylic acid signaling.ResultsWe expressed the gene encoding AtPAD4 in soybean roots of composite plants to test the ability of AtPAD4 to deter plant parasitic nematode development. The transformed roots were challenged with two different plant parasitic nematode genera represented by soybean cyst nematode (SCN; Heterodera glycines) and root-knot nematode (RKN; Meloidogyne incognita). Expression of AtPAD4 in soybean roots decreased the number of mature SCN females 35 days after inoculation by 68 percent. Similarly, soybean roots expressing AtPAD4 exhibited 77 percent fewer galls when challenged with RKN.ConclusionsOur experiments show that AtPAD4 can be used in an economically important crop, soybean, to provide a measure of resistance to two different genera of nematodes.

【 授权许可】

Unknown   
© Youssef et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.