Sake lees extract improves hepatic lipid accumulation in high fat diet-fed mice

Lipids in Health and Disease

Research

Masato Hoshi¹  Yasuko Yamamoto²  Takuya Matsumoto³  Hisako Kubo³  Kuniaki Saito⁴  Yoji Hata⁵  Hiroko Tsutsumi⁵  Shin Oura⁵  Motoko Irie⁵ 

[1] Department of Biochemical and Analytical Sciences, Fujita Health University Graduate School of Health Sciences, 1-98 Dengakugakubo, Kutsukakecho, 470-1192, Toyoake, Aichi, Japan;Department of Disease Control and Prevention, Fujita Health University Graduate School of Health Sciences, 1-98 Dengakugakubo, Kutsukakecho, 470-1192, Toyoake, Aichi, Japan;Human Health Sciences, Graduate School of Medicine and Faculty of Medicine, Kyoto University, 54 Kawaharacho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan;Human Health Sciences, Graduate School of Medicine and Faculty of Medicine, Kyoto University, 54 Kawaharacho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan;Department of Disease Control and Prevention, Fujita Health University Graduate School of Health Sciences, 1-98 Dengakugakubo, Kutsukakecho, 470-1192, Toyoake, Aichi, Japan;Research Institute, Gekkeikan Sake Co. Ltd., 247 Minamihamcho, Fushimi, 612-8385, Kyoto, Japan;

关键词: Sake lees extract;    NAFLD;    Lipid accumulation;    Insulin resistance;   

DOI  :  10.1186/s12944-017-0501-y

 received in 2016-12-08, accepted in 2017-05-25,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundNonalcoholic fatty liver disease (NAFLD) is increasing worldwide as one of the leading causes of chronic liver disease. Sake lees (SL) are secondary products of sake manufacturing and are considered to have beneficial effects on human health. To investigate these effects, we used high fat diet (HFD)-fed mice treated with or without the SL extract.MethodMice were the HFD ad libitum for 8 weeks and were administered 500 μL of distilled water with or without the SL extract (350 mg/mL) by a feeding needle daily for the last 4 weeks. Food intake, body weight, and liver weight were measured. Triacylglycerol content and the mRNA and protein expression levels of various lipid and glucose metabolism-related genes were determined in liver tissues. The levels of triglyceride, free fatty acids, glucose, insulin, and liver cell damage markers were determined in serum. Fatty acid-induced lipid accumulation in HepG2 cells was assessed in the presence or absence of the SL extract.ResultsMice fed a HFD and treated with the SL extract demonstrated a significant reduction in hepatic lipid accumulation and mRNA and protein levels of peroxidome proliferator-activated receptor γ (PPARγ), PPARα, CD36, and phosphoenolpyruvate carboxykinase 1 in the liver, while the SL extract did not affect body weight and food intake. Moreover, insulin resistance and hepatic inflammation in HFD-fed mice improved after administration of the SL extract. In HepG2 cells, the SL extract suppressed fatty acid-induced intracellular lipid accumulation.ConclusionsThese findings suggest that treatment with the SL extract could potentially reduce the risk of NAFLD development, and that the SL extract may be clinically useful for the treatment of NAFLD.

【 授权许可】

CC BY   
© The Author(s). 2017