Development and evaluation of mosquito-electrocuting traps as alternatives to the human landing catch technique for sampling host-seeking malaria vectors

Malaria Journal

Research

Nosrat Mirzai¹  Nicodem J. Govella²  Gerry F. Killeen³  Paul C. D. Johnson⁴  Heather M. Ferguson⁴  Katharina Kreppel⁵  Deodatus V. Maliti⁶ 

[1] Bioelectronics Unit, University of Glasgow, Graham Kerr Building, G12 8QQ, Glasgow, UK;Environmental Health and Ecological Sciences, Ifakara Health Institute, PO Box 78373, Kiko Avenue, Mikocheni B, Dar es Salaam, Tanzania;Environmental Health and Ecological Sciences, Ifakara Health Institute, PO Box 78373, Kiko Avenue, Mikocheni B, Dar es Salaam, Tanzania;Department of Vector Biology, Liverpool School of Tropical Medicine, Pembroke Place, L3 5QA, Liverpool, UK;Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Graham Kerr Building, G12 8QQ, Glasgow, UK;Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Graham Kerr Building, G12 8QQ, Glasgow, UK;Environmental Health and Ecological Sciences, Ifakara Health Institute, PO Box 78373, Kiko Avenue, Mikocheni B, Dar es Salaam, Tanzania;Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Graham Kerr Building, G12 8QQ, Glasgow, UK;Environmental Health and Ecological Sciences, Ifakara Health Institute, PO Box 78373, Kiko Avenue, Mikocheni B, Dar es Salaam, Tanzania;School of Life Sciences, Nelson Mandela African Institute of Science and Technology Tanzania, PO Box 447, Arusha, Tanzania;

关键词: Mosquito electrocuting trap;    Human landing catch;    Mosquito behaviour;    Vector sampling tools;    Outdoor biting;    Malaria;    Anopheles arabiensis;    Anopheles gambiae s.l;    Anopheles funestus;   

DOI  :  10.1186/s12936-015-1025-4

 received in 2015-10-22, accepted in 2015-12-02,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe human landing catch (HLC) is the gold standard method for sampling host-seeking malaria vectors. However, the HLC is ethically questionable because it requires exposure of humans to potentially infectious mosquito bites.MethodsTwo exposure-free methods for sampling host-seeking mosquitoes were evaluated using electrocuting surfaces as potential replacements for HLC: (1) a previously evaluated, commercially available electrocuting grid (CA-EG) designed for killing flies, and (2) a custom-made mosquito electrocuting trap (MET) designed to kill African malaria vectors. The MET and the CA-EG were evaluated relative to the HLC in a Latin Square experiment conducted in the Kilombero Valley, Tanzania. The sampling consistency of the traps across the night and at varying mosquito densities was investigated. Estimates of the proportion of mosquitoes caught indoors (Pi), proportion of human exposure occurring indoors (πi), and proportion of mosquitoes caught when most people are likely to be indoors (Pfl) were compared for all traps.ResultsWhereas the CA-EG performed poorly (<10 % of catch of HLC), sampling efficiency of the MET for sampling Anopheles funestuss.l. was indistinguishable from HLC indoors and outdoors. For Anopheles gambiae s.l., sampling sensitivity of MET was 20.9 % (95 % CI 10.3–42.2) indoors and 58.5 % (95 % CI 32.2–106.2) outdoors relative to HLC. There was no evidence of density-dependent sampling by the MET or CA-EG. Similar estimates of Pi were obtained for An. gambiaes.l. and An. funestus s.l. from all trapping methods. The proportion of mosquitoes caught when people are usually indoors (Pfl) was underestimated by the CA-EG and MET for An. gambiae s.l., but similar to the HLC for An. funestus. Estimates of the proportion of human exposure occurring indoors (πi) obtained from the CA-EG and MET were similar to the HLC for An. gambiae s.l., but overestimated for An. funestus.ConclusionsThe MET showed promise as an outdoor sampling tool for malaria vectors where it achieved >50 % sampling sensitivity relative to the HLC. The CA-EG had poor sampling sensitivity outdoors and inside. With further modification, the MET could provide an efficient and safer alternative to the HLC for the surveillance of mosquito vectors outdoors.

【 授权许可】

CC BY   
© Maliti et al. 2015