Neuroactivity of detonation nanodiamonds: dose-dependent changes in transporter-mediated uptake and ambient level of excitatory/inhibitory neurotransmitters in brain nerve terminals

Journal of Nanobiotechnology

Research

Tatiana Borisova¹  Maxim Galkin¹  Marina Dudarenko¹  Natalia Pozdnyakova¹  Artem Pastukhov¹  Arsenii Borysov¹ 

[1] Department of Neurochemistry, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, 9 Leontovicha Street, 01601, Kiev, Ukraine;

关键词: Nanodiamonds;    Glutamate;    γ-aminobutyric acid;    Na-dependent uptake;    The extracellular level;    Exocytosis;    Brain nerve terminals;   

DOI  :  10.1186/s12951-016-0176-y

 received in 2016-01-26, accepted in 2016-03-18,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundNanodiamonds are one of the most perspective nano-sized particles with superb physical and chemical properties, which are mainly composed of carbon sp3 structures in the core with sp2 and disorder/defect carbons on the surface. The research team recently demonstrated neuromodulatory properties of carbon nanodots with other than nanodiamonds hybridization types, i.e., sp2 hybridized graphene islands and diamond-like sp3 hybridized elements.ResultsIn this study, neuroactive properties of uncoated nanodiamonds produced by detonation synthesis were assessed basing on their effects on transporter-mediated uptake and the ambient level of excitatory and inhibitory neurotransmitters, glutamate and γ-aminobutyric acid (GABA), in isolated rat brain nerve terminals. It was shown that nanodiamonds in a dose-dependent manner attenuated the initial velocity of Na+-dependent transporter-mediated uptake and accumulation of l-[14C]glutamate and [3H]GABA by nerve terminals and increased the ambient level of these neurotransmitters. Also, nanodiamonds caused a weak reduction in acidification of synaptic vesicles and depolarization of the plasma membrane of nerve terminals.ConclusionsTherefore, despite different types of hybridization in nanodiamonds and carbon dots, they exhibit very similar effects on glutamate and GABA transport in nerve terminals and this common feature of both nanoparticles is presumably associated with their nanoscale size. Observed neuroactive properties of pure nanodiamonds can be used in neurotheranostics for simultaneous labeling/visualization of nerve terminals and modulation of key processes of glutamate- and GABAergic neurotransmission. In comparison with carbon dots, wider medical application involving hypo/hyperthermia, external magnetic fields, and radiolabel techniques can be perspective for nanodiamonds.

【 授权许可】

CC BY   
© Pozdnyakova et al. 2016