DNA-nanostructure-assembly by sequential spotting

Journal of Nanobiotechnology

Research

Peter E Nielsen¹  Ralph Hölzel²  Frank F Bier³  Michael Breitenstein³ 

[1] Department of Cellular and Molecular Medicine, Health Science Faculty University of Copenhagen Blegdamsvej 3c, DK-2100 N, Copenhagen, Denmark;Fraunhofer Institute for Biomedical Engineering Department of Nanobiotechnology and Nanomedicine Am Mühlenberg 13, 14476, Potsdam, Germany;Fraunhofer Institute for Biomedical Engineering Department of Nanobiotechnology and Nanomedicine Am Mühlenberg 13, 14476, Potsdam, Germany;University of Potsdam Institute for Biochemistry and Biology Karl-Liebknecht-Str. 24-25, 14476, Potsdam, Germany;

关键词: Atomic Force Microscope;    Peptide Nucleic Acid;    Persistence Length;    Adapter Oligonucleotide;    High Fluorescence Signal;   

DOI  :  10.1186/1477-3155-9-54

 received in 2011-09-15, accepted in 2011-11-18,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe ability to create nanostructures with biomolecules is one of the key elements in nanobiotechnology. One of the problems is the expensive and mostly custom made equipment which is needed for their development. We intended to reduce material costs and aimed at miniaturization of the necessary tools that are essential for nanofabrication. Thus we combined the capabilities of molecular ink lithography with DNA-self-assembling capabilities to arrange DNA in an independent array which allows addressing molecules in nanoscale dimensions.ResultsFor the construction of DNA based nanostructures a method is presented that allows an arrangement of DNA strands in such a way that they can form a grid that only depends on the spotted pattern of the anchor molecules. An atomic force microscope (AFM) has been used for molecular ink lithography to generate small spots. The sequential spotting process allows the immobilization of several different functional biomolecules with a single AFM-tip. This grid which delivers specific addresses for the prepared DNA-strand serves as a two-dimensional anchor to arrange the sequence according to the pattern. Once the DNA-nanoarray has been formed, it can be functionalized by PNA (peptide nucleic acid) to incorporate advanced structures.ConclusionsThe production of DNA-nanoarrays is a promising task for nanobiotechnology. The described method allows convenient and low cost preparation of nanoarrays. PNA can be used for complex functionalization purposes as well as a structural element.

【 授权许可】

CC BY   
© Breitenstein et al; licensee BioMed Central Ltd. 2011