Lentiviral vector-mediated RBM5 overexpression downregulates EGFR expression in human non-small cell lung cancer cells

World Journal of Surgical Oncology

Research

Lijing Zhao¹  Hong Liang²  Jinzhi Yin³  Jie Zhang³  Ke Wang³  Zhenzhong Su³  Ranwei Li⁴ 

[1] Department of Pathophysiology, Norman Bethune College of Medicine of Jilin University, No.126 Xinmin Street, 130021, Chaoyang District, Changchun, Jilin, China;Department of Respiratory Medicine, Changchun General Hospital, No.1810 Renmin Street, 130021, Nanguan District, Changchun, Jilin, China;Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, No.218 Ziqiang Street, 130041, Nanguan District, Changchun, Jilin, China;Department of Urinary Surgery, The Second Affiliated Hospital of Jilin University, No.218 Ziqiang Street, 130041, Nanguan District, Changchun, Jilin, China;

关键词: RNA binding motif 5;    Epidermal growth factor receptor;    Non-small cell lung cancer;    Lentiviral vector;    A549;    Xenograft mice model;   

DOI  :  10.1186/1477-7819-12-367

 received in 2014-08-26, accepted in 2014-11-18,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundRNA binding motif 5 (RBM5) is a tumor suppressor gene that modulates apoptosis through the regulation of alternative splicing of apoptosis-related genes. Our previous studies suggested that RBM5 expression was negatively correlated with the expression of epidermal growth factor receptor (EGFR) in non-small cell lung cancer (NSCLC) tissues. This study was aimed at determining whether RBM5 is able to regulate EGFR expression.MethodsBoth in vitro and in vivo studies were carried out to determine the effect of RBM5 on the expression of EGFR. Lentiviral vector-mediated RBM5 overexpression was employed in lung adenocarcinoma cell line A549. A549 xenograft mice were treated with recombinant RBM5 plasmid carried by attenuated Salmonella typhi Ty21a. Real-time quantitative polymerase chain reaction and Western blot were carried out to detect RBM5 and EGFR expression.ResultsBoth in vivo and in vitro studies indicated that the expression of EGFR mRNA and protein was decreased significantly in the RBM5 overexpression group compared to control groups as shown by real-time PCR and Western blot analysis. We identified that RBM5 overexpression inhibited EGFR expression both in A549 cells and in A549 xenograft mice model.ConclusionsOur study demonstrated that EGFR expression is regulated by RBM5 in lung adenocarcinomas cells either in a direct or indirect way, which might be meaningful with regards to target therapy in lung cancer.

【 授权许可】

Unknown   
© Su et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.