FOXO1, PXK, PYCARD and SAMD9L are differentially expressed by fibroblast-like cells in equine synovial membrane compared to joint capsule

BMC Veterinary Research

Research Article

Lise Charlotte Berg¹  Line Nymann Thomsen¹  Preben Dybdahl Thomsen²  Alison Downing³  Richard Talbot³ 

[1] Department of Veterinary Clinical Sciences, University of Copenhagen, Hoejbakkegaards alle 5, 2630, Taastrup, Denmark;Department of Veterinary and Animal Sciences, University of Copenhagen, Groennegaardsvej 7, 1870, Frederiksberg C, Denmark;Edinburgh Genomics, Ashworth Laboratories, University of Edinburgh, EH9 3FL, Edinburgh, UK;

关键词: Synovial membrane;    Fibroblast-like synoviocytes;    FOXO1;    PXK;    PYCARD;    SAMD9L;    Equine;    Marker;   

DOI  :  10.1186/s12917-017-1003-x

 received in 2015-11-17, accepted in 2017-03-28,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe synovial membrane lines the luminal side of the joint capsule in synovial joints. It maintains joint homeostasis and plays a crucial role in equine joint pathology. When trauma or inflammation is induced in a joint, the synovial membrane influences progression of joint damage. Equine synovial membrane research is hampered by a lack of markers of fibroblast-like synoviocytes (FLS) to distinguish FLS from other fibroblast-like cells in musculoskeletal connective tissues. The aim of this study is to identify potential FLS markers of the equine synovial membrane using microarray to compare between gene expression in equine synovial membrane and the joint capsule in metacarpophalangeal joints.ResultsMicroarray analysis of tissues from 6 horses resulted in 1167 up-regulated genes in synovial membrane compared with joint capsule. Pathway analysis resulted in 241 candidate genes. Of these, 15 genes were selected for further confirmation as genes potentially expressed by fibroblast-like synoviocytes. Four genes: FOXO1, PXK, PYCARD and SAMD9L were confirmed in 9 horses by qPCR as differentially expressed in synovial membrane compared to joint capsule.ConclusionsIn conclusion, FOXO1, PXK, PYCARD and SAMD9L were confirmed as differentially expressed in synovial membrane compared to joint capsule. These four genes are potential markers of fibroblast-like synoviocytes of the synovial membrane. As these genes are overexpressed in synovial membrane compared to joint capsule, these genes could shed light on synovial membrane physiology and its role in joint disease.

【 授权许可】

CC BY   
© The Author(s). 2017