Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots

Proteome Science

Research

Yang Liu¹  Hong Tian¹  Mingxin Chen¹  Junbo Xiong¹  Heshan Zhang¹  Qingchuan Yang²  Yan Sun³ 

[1] Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal and Veterinary Science, Hubei Academy of Agricultural Science, Yaoyuan 1, Hongshan, 430017, Wuhan, Hubei, China;Institute of Animal Science, Chinese Academy of Agricultural Science, West Road 2, Yuan Ming Yuan, 100193, Beijing, China;Institute of Grassland Science, China Agricultural University, 2 West Road, Yuan Ming Yuan, 100193, Beijing, China;

关键词: NaCl stress;    Medicago sativa;    Two-dimensional electrophoresis;    Differentially abundant proteins;   

DOI  :  10.1186/s12953-017-0127-z

 received in 2017-06-08, accepted in 2017-10-25,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAlfalfa (Medicago sativa) is the most extensively cultivated forage legume in the world, and salinity stress is the most problematic environmental factors limiting alfalfa production. To evaluate alfalfa tissue variations in response to salt stress, comparative physiological and proteomic analyses were made of salt responses in the roots and shoots of the alfalfa.MethodA two-dimensional gel electrophoresis (2-DE)-based proteomic technique was employed to identify the differentially abundant proteins (DAPs) from salt-treated alfalfa roots and shoots of the salt tolerance cultivars Zhongmu No 1 cultivar, which was subjected to a range of salt stress concentrations for 9 days. In parallel, REL, MAD and H2O2 contents, and the activities of antioxidant enzymes of shoots and roots were determinand.ResultTwenty-seven spots in the shoots and 36 spots in the roots that exhibited showed significant abundance variations were identified by MALDI-TOF-TOF MS. These DAPs are mainly involved in the biological processes of photosynthesis, stress and defense, carbohydrate and energy metabolism, second metabolism, protein metabolism, transcriptional regulation, cell wall and cytoskeleton metabolism, ion transpor, signal transduction. In parallel, physiological data were correlated well with our proteomic results. It is worth emphasizing that some novel salt-responsive proteins were identified, such as CP12, pathogenesis-related protein 2, harvest-induced protein, isoliquiritigenin 2′-O-methyltransferase. qRT-PCR was used to study the gene expression levels of the four above-mentioned proteins; four patterns are consistent with those of induced protein.ConclusionThe primary mechanisms underlying the ability of alfalfa seedlings to tolerate salt stress were photosynthesis, detoxifying and antioxidant, secondary metabolism, and ion transport. And it also suggests that the different tissues responded to salt-stress in different ways.

【 授权许可】

CC BY   
© The Author(s). 2017