Comparative study of anti-angiogenic activities of luteolin, lectin and lupeol biomolecules

Journal of Translational Medicine

Research

Renu Sharma¹  Dhiraj Kumar¹  Saurabh Kumar Jha²  Rashmi K. Ambasta²  Niraj Kumar Jha²  Pravir Kumar³ 

[1] Department of Biotechnology, Delhi Technological University (Former Delhi College of Engineering), Delhi, India;Department of Biotechnology, Delhi Technological University (Former Delhi College of Engineering), Delhi, India;School of Biosciences and Technology, Vellore Institute of Technology, University (VITU), Vellore, India;Department of Biotechnology, Delhi Technological University (Former Delhi College of Engineering), Delhi, India;School of Biosciences and Technology, Vellore Institute of Technology, University (VITU), Vellore, India;Neurology Department, Adjunct Faculty, Tufts University School of Medicine, Boston, MA, USA;

关键词: CAM assay;    Flavonoids;    HT-29 cell;    Anti-angiogenesis;    Luteolin;    Lupeol;    Lectin;   

DOI  :  10.1186/s12967-015-0665-z

 received in 2015-04-08, accepted in 2015-09-08,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAngiogenesis is a hallmark feature in the initiation, progression and growth of tumour. There are various factors for promotion of angiogenesis on one hand and on the other hand, biomolecules have been reported to inhibit cancer through anti-angiogenesis mechanism. Biomolecules, for instance, luteolin, lectin and lupeol are known to suppress cancer. This study aims to compare and evaluate the biomolecule(s) like luteolin, lupeol and lectin on CAM assay and HT-29 cell culture to understand the efficacy of these drugs.MethodThe biomolecules have been administered on CAM assay, HT-29 cell culture, cell migration assay. Furthermore, bioinformatics analysis of the identified targets of these biomolecules have been performed.ResultLuteolin has been found to be better in inhibiting angiogenesis on CAM assay in comparison to lupeol and lectin. In line with this study when biomolecules was administered on cell migration assay via scratch assay method. We provided evidence that Luteolin was again found to be better in inhibiting HT-29 cell migration. In order to identify the target sites of luteolin for inhibition, we used software analysis for identifying the best molecular targets of luteolin. Using software analysis best target protein molecule of these biomolecules have been identified. VEGF was found to be one of the target of luteolin. Studies have found several critical point mutation in VEGF A, B and C. Hence docking analysis of all biomolecules with VEGFR have been performed. Multiple allignment result have shown that the receptors are conserved at the docking site.ConclusionTherefore, it can be concluded that luteolin is not only comparatively better in inhibiting blood vessel in CAM assay, HT-29 cell proliferation and cell migration assay rather the domain of VEGFR is conserved to be targeted by luteolin, lupeol and lectin.

【 授权许可】

CC BY   
© Ambasta et al. 2015