Non-canonical regulation of phosphatidylinositol 3-kinase gamma isoform activity in retinal rod photoreceptor cells

Cell Communication and Signaling

Research

Vivek K Gupta¹  Ammaji Rajala¹  Raju VS Rajala² 

[1] Department of Ophthalmology, University of Oklahoma Health Sciences Center, 608 Stanton L. Young Boulevard, 73104, Oklahoma City, OK, USA;Dean A. McGee Eye Institute, 608 Stanton L. Young Boulevard, 73104, Oklahoma City, OK, USA;Department of Ophthalmology, University of Oklahoma Health Sciences Center, 608 Stanton L. Young Boulevard, 73104, Oklahoma City, OK, USA;Department of Physiology, University of Oklahoma Health Sciences Center, 608 Stanton L. Young Boulevard, 73104, Oklahoma City, OK, USA;Department of Cell Biology, University of Oklahoma Health Sciences Center, 608 Stanton L. Young Boulevard, 73104, Oklahoma City, OK, USA;Dean A. McGee Eye Institute, 608 Stanton L. Young Boulevard, 73104, Oklahoma City, OK, USA;

关键词: Cyclic nucleotide-gated channel;    Phosphatidylinositol 3-kinase gamma;    Photoreceptor outer segments;    Ras-associating domain;    Ras-like domain;   

DOI  :  10.1186/s12964-015-0087-9

 received in 2014-11-23, accepted in 2015-01-20,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPhosphatidylinositol 3-Kinases (PI3Ks) are a family of lipid kinases that phosphorylate the D3-hydroxyls of the inositol ring of phosphoinositides, and are responsible for coordinating a diverse range of cellular functions. A canonical pathway of activation of PI3Ks through the interaction of RA-domain with Ras proteins has been well established. In retinal photoreceptors, we have identified a non-canonical pathway of PI3Kγ activation through the interaction of its RA-domain with a putative Ras-like domain (RLD) in alpha subunit of cyclic nucleotide-gated channel (CNGA1) in retinal rod photoreceptors.ResultsThe interaction between PI3Kγ and CNGA1 does not appear to play a role in regulation of CNG channel activity, but PI3Kγ uses CNGA1 as an anchoring module to achieve close proximity to its substrate to generate D3-phosphoinositides.ConclusionsOur studies suggest a functional non-canonical PI3Kγ activation in retinal rod photoreceptor cells.

【 授权许可】

Unknown   
© Gupta et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.