Fenofibrate decreases the bone quality by down regulating Runx2 in high-fat-diet induced Type 2 diabetes mellitus mouse model

Lipids in Health and Disease

Research

Tianshu Shi¹  Siyu Shen¹  Xiaobo Zhu¹  Yong Shi¹  Ke Lu¹  Xianglin Liu¹  Kaijia Zhang¹  Huajian Teng²  Qing Jiang²  Chaojun Li³  Qiaoli Tang³  Bin Xue⁴ 

[1] Department of Sports Medicine and Adult Reconstructive Surgery, Drum Tower Hospital, School of Medicine, Nanjing University, No. 321 Zhongshan Road, 210008, Nanjing, People’s Republic of China;Department of Sports Medicine and Adult Reconstructive Surgery, Drum Tower Hospital, School of Medicine, Nanjing University, No. 321 Zhongshan Road, 210008, Nanjing, People’s Republic of China;Joint Research Center for Bone and Joint Disease, Model Animal Research Center (MARC), Nanjing University, 210093, Nanjing, China;State Key Laboratory of Pharmaceutical Biotechnology and Jiangsu Key Laboratory of Molecular Medicine and School of Medicine, Nanjing University, No. 22 Hankou Road, Gulou District, 210093, Nanjing, Jiangsu Province, China;State Key Laboratory of Pharmaceutical Biotechnology and Jiangsu Key Laboratory of Molecular Medicine and School of Medicine, Nanjing University, No. 22 Hankou Road, Gulou District, 210093, Nanjing, Jiangsu Province, China;State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009, Nanjing, China;Liver Disease Collaborative Research Platform of Medical School of Nanjing University, 210093, Nanjing, China;

关键词: Fenofibrate;    Bone quality;    Runx2;    Type 2 diabetes mellitus;   

DOI  :  10.1186/s12944-017-0592-5

 received in 2017-08-26, accepted in 2017-10-04,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThis study is to investigate the effect of fenofibrate on the bone quality of Type 2 diabetes mellitus (T2DM) mouse model.MethodsT2DM mouse model was induced by high-fat-diet, and the mice were treated with fenofibrate (100 mg/kg) (DIO-FENO) or PBS (DIO-PBS) for 4 weeks. The bone microstructure and biomechanical properties of femora were analyzed by micro-CT and 3-Point bending test. The protein expression was detected by immunohistochemical staining and Western blot. The cell apoptosis was evaluated by TUNEL staining. The Bcl2, caspase 3, and osteoblast marker genes were detected by RT-qPCR.ResultsThe biomechanical properties of bones from DIO-FENO group were significantly lower than those in the control and DIO-PBS groups. Besides, the trabecular number was lower than those of the other groups, though the cortical porosity was decreased compared with that of DIO-PBS group because of the increase of apoptotic cells. The expression of osteocalcin and collagen I were decreased after treatment with fenofibrate in T2DM mice. Moreover, the cell viability was decreased after treated with different concentrations of fenofibrate, and the expression of Runx2 decreased after treated with high dose of fenofibrate.ConclusionFenofibrate decreases the bone quality of T2DM mice through decreasing the expression of collagen I and osteocalcin, which may be resulted from the down regulation of Runx2 expression.

【 授权许可】

CC BY   
© The Author(s). 2017