Integrin expression profiling identifies integrin alpha5 and beta1 as prognostic factors in early stage non-small cell lung cancer

Molecular Cancer

Research

Robert-Jan van Suylen¹  Arjan W Griffioen²  Victor L Thijssen³  Vivian van den Boogaart⁴  Anne-Marie C Dingemans⁴  Bettine A Vosse⁵ 

[1] Department of Pathology, Maastricht University Medical Center, P. Debyeplein 25, 6229 HX, Maastricht, The Netherlands;Department of Pathology, Maastricht University Medical Center, P. Debyeplein 25, 6229 HX, Maastricht, The Netherlands;Angiogenesis laboratory, Department of Medical Oncology, VU University medical center, De Boelelaan 1118, 1081 HV, Amsterdam, The Netherlands;Department of Pathology, Maastricht University Medical Center, P. Debyeplein 25, 6229 HX, Maastricht, The Netherlands;Angiogenesis laboratory, Department of Medical Oncology, VU University medical center, De Boelelaan 1118, 1081 HV, Amsterdam, The Netherlands;Angiogenesis laboratory, Department of Radiotherapy, VU University medical center, De Boelelaan 1118, 1081 HV, Amsterdam, The Netherlands;Department of Pulmonology, Maastricht University Medical Center, P. Debyeplein 25, 6229 HX, Maastricht, The Netherlands;Department of Pulmonology, Maastricht University Medical Center, P. Debyeplein 25, 6229 HX, Maastricht, The Netherlands;Department of Pathology, Maastricht University Medical Center, P. Debyeplein 25, 6229 HX, Maastricht, The Netherlands;

关键词: Integrin;    NSCLC Patient;    Integrin Expression;    Large Cell Carcinoma;    Integrin Alpha5;   

DOI  :  10.1186/1476-4598-9-152

 received in 2009-12-07, accepted in 2010-06-17,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundSelection of early stage non-small cell lung cancer patients with a high risk of recurrence is warranted in order to select patients who will benefit from adjuvant treatment strategies. We evaluated the prognostic value of integrin expression profiles in a retrospective study on frozen primary tumors of 68 patients with early stage non-small cell lung cancer.MethodsA retrospective study was performed on frozen primary tumors of 68 early stage non-small cell lung cancer patients with a follow up of at least 10 years. From all tumor tissues, RNA was isolated and reverse transcribed into cDNA. qPCR was used to generate mRNA expression profiles including integrins alpha1, 2, 3, 4, 5, 6, 7, 11, and V as well as integrins beta1, 3, 4, 5, 6, and 8.ResultsThe expression levels of integrins alpha5, beta1 and beta3 predicted overall survival and disease free survival in early stage NSCLC patients. There was no association between integrin expression and lymph node metastases. Comparison between the histological subtypes revealed a distinct integrin signature for squamous cell carcinoma while the profiles of adenocarcinoma and large cell carcinoma were largely the same.ConclusionIntegrin expression in NSCLC is important for the development and behavior of the tumor and influences the survival of the patient. Determining the integrin expression profile might serve as a tool in predicting the prognosis of individual patients.

【 授权许可】

CC BY   
© Dingemans et al; licensee BioMed Central Ltd. 2010