A major quantitative trait locus conferring adult plant partial resistance to crown rust in oat

BMC Plant Biology

Research Article

James Chong¹  Belaghihalli N Gnanesh¹  Jim G Menzies¹  Curt A McCartney¹  Gang Chen¹  Jennifer W Mitchell Fetch¹  H Randy Kutcher²  Aaron D Beattie²  Peter E Eckstein²  Yang Lin²  Eric W Jackson³ 

[1] Agriculture and Agri-Food Canada, Cereal Research Centre, 101 Route 100, R6M 1Y5, Morden, MB, Canada;Crop Development Centre/Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, S7N 5A8, Saskatoon, SK, Canada;General Mills Agriculture Research, 150 N. Research Campus Dr, 28081, Kannapolis, NC, USA;

关键词: Crown rust;    Puccinia coronata;    Oat;    Avena sativa;    Adult plant resistance;    Partial resistance;    SNP;    QTL;   

DOI  :  10.1186/s12870-014-0250-2

 received in 2014-07-10, accepted in 2014-09-18,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundCrown rust, caused by Puccinia coronata f. sp. avenae, is the most important disease of oat worldwide. Adult plant resistance (APR), based upon partial resistance, has proven to be a durable rust management strategy in other cereal rust pathosystems. The crown rust APR in the oat line MN841801 has been effective for more than 30 years. The genetic basis of this APR was studied under field conditions in three recombinant inbred line (RIL) populations: 1) AC Assiniboia/MN841801, 2) AC Medallion/MN841801, and 3) Makuru/MN841801. The populations were evaluated for crown rust resistance with the crown rust isolate CR251 (race BRBB) in multiple environments. The 6 K oat and 90 K wheat Illumina Infinium single nucleotide polymorphism (SNP) arrays were used for genotyping the AC Assiniboia/MN841801 population. KASP assays were designed for selected SNPs and genotyped on the other two populations.ResultsThis study reports a high density genetic linkage map constructed with oat and wheat SNP markers in the AC Assiniboia/MN841801 RIL population. Most wheat SNPs were monomorphic in the oat population. However the polymorphic wheat SNPs could be scored accurately and integrated well into the linkage map. A major quantitative trait locus (QTL) on oat chromosome 14D, designated QPc.crc-14D, explained up to 76% of the APR phenotypic variance. This QTL is flanked by two SNP markers, GMI_GBS_90753 and GMI_ES14_c1439_83. QPc.crc-14D was validated in the populations AC Medallion/MN841801 and Makuru/MN841801.ConclusionsWe report the first APR QTL in oat with a large and consistent effect. QPc.crc-14D was statistically significant in all environments tested in each of the three oat populations. QPc.crc-14D is a suitable candidate for use in marker-assisted breeding and also an excellent target for map-based cloning. This is also the first study to use the 90 K wheat Infinium SNP array on oat for marker development and comparative mapping. The Infinium SNP array is a useful tool for saturating oat maps with markers. Synteny with wheat suggests that QPc.crc-14D is orthologous with the stripe rust APR gene Yr16 in wheat.

【 授权许可】

Unknown   
© Lin et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.