Methionine enkephalin(MENK) upregulated memory T cells in anti-influenza response

BMC Immunology

Research

Miao Miao¹  Xiaonan Wang¹  Fengping Shan²  Zifeng Xie³  Wenrui Fu³  Jing Tian⁴  Xiaodong Yu⁵ 

[1] Biostax Inc., 1317 Edgewater Dr., Ste 4882, 32804, Orlando, FL, USA;Department of Immunology, School of Basic Medical Science, China Medical University, 110122, Shenyang, China;Department of Immunology, School of Basic Medical Science, Jinzhou Medical University, 121001, Jinzhou, China;Department of Immunology, School of Basic Medical Science, Jinzhou Medical University, 121001, Jinzhou, China;Department of Immunology, School of Basic Medical Science, China Medical University, 110122, Shenyang, China;Department of Nursing, The First Affiliated Hospital of Jinzhou Medical University, 121001, Jinzhou, China;

关键词: Methionine enkephalin;    Influenza A virus;    Opioid receptor;    Antiviral efficacy;    Memory T cell;   

DOI  :  10.1186/s12865-023-00573-0

 received in 2023-03-30, accepted in 2023-09-20,  发布年份 2023

来源: Springer

【 摘 要 】

Novel prophylactic drugs and vaccination strategies for protection against influenza virus should induce specific effector T-cell immune responses in pulmonary airways and peripheral lymphoid organs. Designing approaches that promote T-cell-mediated responses and memory T-cell differentiation would strengthen host resistance to respiratory infectious diseases. The results of this study showed that pulmonary delivery of MENK via intranasal administration reduced viral titres, upregulated opioid receptor MOR and DOR, increased the proportions of T-cell subsets including CD8+ T cells, CD8+ TEM cells, NP/PA-effector CD8+ TEM cells in bronchoalveolar lavage fluid and lungs, and CD4+/CD8+ TCM cells in lymph nodes to protect mice against influenza viral challenge. Furthermore, we demonstrated that, on the 10th day of infection, the proportions of CD4+ TM and CD8+ TM cells were significantly increased, which meant that a stable TCM and TEM lineage was established in the early stage of influenza infection. Collectively, our data suggested that MENK administered intranasally, similar to the route of natural infection by influenza A virus, could exert antiviral activity through upregulating T-cell-mediated adaptive immune responses against influenza virus.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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