Evaluating decontamination protocols for the isolation of Mycobacterium ulcerans from swabs

BMC Microbiology

Research Article

Enid Owusu¹  Amos Akumwena²  Elizabeth Bannerman²  Mercy J. Newman²  Gerd Pluschke³ 

[1] Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana;Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana;Swiss Tropical Institute, Basel, Switzerland;

关键词: Ghana;    Buruli ulcer;    Mycobacterium ulcerans;    Decontamination;    Cetyl pyridinium chloride;    Oxalic acid;   

DOI  :  10.1186/s12866-016-0918-x

 received in 2016-02-22, accepted in 2016-12-20,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMycobacterium ulcerans (M. ulcerans) is the causative agent of Buruli Ulcer (BU) disease. In order to inhibit the growth of the microbial contaminants during culture of M. ulcerans, it is necessary to decontaminate BU samples with effective chemical agents. This study aimed at investigating some selected chemicals as potential decontamination agents for the isolation of M. ulcerans from swabs.ResultsPovidone iodine at 0.5 and 1% exhibited the lowest contamination and recovery rate for microbial contaminants and M. ulcerans. The most effective decontamination method was the protocol using 2% cetylpyridinium chloride/4% sodium chloride (recovery rate = 53%, contamination rate = 14%). The observed difference between the recovery rate of 2% CPC/4% NaC and the other protocols was however not statistically significant (p = 0.76).ConclusionsTwo percent (2%) cetylpyridinium chloride/4% sodium chloride can be conveniently used as an alternative decontamination method for the isolation of M. ulcerans from swabs.

【 授权许可】

CC BY   
© The Author(s). 2017