Mode of antiviral action of silver nanoparticles against HIV-1

Journal of Nanobiotechnology

Research

Nilda V Ayala-Nuñez¹  Cristina Rodriguez-Padilla¹  Liliana Ixtepan-Turrent¹  Humberto H Lara¹ 

[1] Laboratorio de Inmunología y Virología, Departamento de Microbiología e Inmunología, Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Mexico;

关键词: Silver Nanoparticles;    Indinavir;    Enfuvirtide;    Fusion Inhibitor;    Silver Sulfadiazine;   

DOI  :  10.1186/1477-3155-8-1

 received in 2009-07-21, accepted in 2010-01-20,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundSilver nanoparticles have proven to exert antiviral activity against HIV-1 at non-cytotoxic concentrations, but the mechanism underlying their HIV-inhibitory activity has not been not fully elucidated. In this study, silver nanoparticles are evaluated to elucidate their mode of antiviral action against HIV-1 using a panel of different in vitro assays.ResultsOur data suggest that silver nanoparticles exert anti-HIV activity at an early stage of viral replication, most likely as a virucidal agent or as an inhibitor of viral entry. Silver nanoparticles bind to gp120 in a manner that prevents CD4-dependent virion binding, fusion, and infectivity, acting as an effective virucidal agent against cell-free virus (laboratory strains, clinical isolates, T and M tropic strains, and resistant strains) and cell-associated virus. Besides, silver nanoparticles inhibit post-entry stages of the HIV-1 life cycle.ConclusionsThese properties make them a broad-spectrum agent not prone to inducing resistance that could be used preventively against a wide variety of circulating HIV-1 strains.

【 授权许可】

CC BY   
© Lara et al; licensee BioMed Central Ltd. 2010