Chromosomal structural analysis in carcinoma of the gallbladder

World Journal of Surgical Oncology

Research

VK Shukla¹  Somprakash Basu¹  Ruhi Dixit¹  Prabhat Kumar¹  Ratnakar Tripathi²  Rajnikanth Mishra² 

[1] Department of General Surgery Institute of Medical Sciences, Banaras Hindu University, 221 005, Varanasi, India;Department of Zoology, Faculty of Science, Banaras Hindu University, 221 005, Varanasi, India;

关键词: Lactate dehydrogenase (LDH);    Superoxide dismutase (SOD);    Catalase;    Carcinoma of the gallbladder;   

DOI  :  10.1186/1477-7819-10-198

 received in 2012-05-03, accepted in 2012-09-13,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe etiopathogenesis of gallbladder cancer is still unknown. Both environmental and patient factors have been incriminated in its cause. That it is found in pockets of epidemiological distribution raises an issue of genetic changes associated with it. The aim of this study was to find out the chromosomal changes in gallbladder cancer.MethodsLymphocyte cell culture was carried out on blood of gallbladder cancer patients to determine chromosomal banding abnormalities. Native PAGE was also evaluated to analyze lactate dehydrogenase (LDH), superoxide dismutase (SOD) and catalase enzyme activity from the same blood of gallbladder cancer patients.ResultsOut of 30 gallbladder cancer patients, 4 male showed breakage on the long arm of chromosome 1 while only one male patient showed the translocation from the long arm of chromosome 4 to the long arm of chromosome 6 in a male patient.ConclusionThe aberrations found in our study may suggest underlying genetic predisposition for the development of gallbladder cancer. They can act as a marker for gallbladder cancer, which needs further study.

【 授权许可】

Unknown   
© Dixit et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.