Characterization of Salmonella enterica serovar Enteritidis isolates recovered from blood and stool specimens in Thailand

BMC Microbiology

Research Article

Phattharaporn Chaichana¹  Srirat Pornruangwong¹  Chaiwat Pulsrikarn¹  Rene S Hendriksen²  Christina Aaby Svendsen²  Matthew Mikoleit³  Eija Hyytia-Trees³  Rafiq Ahmed⁴ 

[1] Department of Medical Sciences, WHO National Salmonella and Shigella Center, National Institute of Health, Ministry of Public Health, Tiwanond Road, Amphur Muang, 11000, Nonthaburi, Thailand;Division of Bacterial Genomics and Epidemiology, WHO Collaborating Center for Antimicrobial Resistance in Food borne Pathogens and European Union Reference Laboratory for Antimicrobial Resistance, National Food Institute, Technical University of Denmark, Kemitorvet, Building 204, DK-2800, Kgs. Lyngby, Denmark;Division of Foodborne, Waterborne, and Environmental Diseases; Enteric Diseases Laboratory Branch; Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases, Mail stop CO3, 1600 Clifton Road, 30333, Atlanta, GA, USA;National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, R3E 3R2, Winnipeg, MB, Canada;

关键词: Minimum Inhibitory Concentration;    Colistin;    Nalidixic Acid;    Phage Type;    Thai Patient;   

DOI  :  10.1186/1471-2180-12-92

 received in 2012-01-18, accepted in 2012-05-17,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundBacteremia due to Salmonella spp. is a life-threatening condition and is commonly associated with immune compromise. A 2009 observational study estimated risk factors for the ten most common non-typhoidal Salmonella (NTS) serovars isolated from Thai patients between 2002–2007. In this study, 60.8% of Salmonella enterica serovar Enteritidis isolates (n = 1517) were recovered from blood specimens and infection with Salmonella serovar Enteritidis was a statistically significant risk factor for bacteremia when compared to other NTS serovars. Based on this information, we characterized a subset of isolates collected in 2008 to determine if specific clones were recovered from blood or stool specimens at a higher rate. Twenty blood isolates and 20 stool isolates were selected for antimicrobial resistance testing (MIC), phage typing, PFGE, and MLVA.ResultEight antibiogrammes, seven MLVA types, 14 XbaI/BlnI PFGE pattern combinations, and 11 phage types were observed indicating considerable diversity among the 40 isolates characterized. Composite analysis based on PFGE and MLVA data revealed 22 genotypes. Seven of the genotypes containing two or more isolates were from both stool and blood specimens originating from various months and zones. Additionally, those genotypes were all further discriminated by phage type and/or antibiogramme. Ninety percent of the isolates were ciprofloxacin resistant.ConclusionsThe increased percentage of bloodstream infections as described in the 2009 observational study could not be attributed to a single clone. Future efforts should focus on assessing the immune status of bacteriaemic patients and identifying prevention and control measures, including attribution studies characterizing non-clinical (animal, food, and environmental) isolates.

【 授权许可】

CC BY   
© Hendriksen et al.; licensee BioMed Central Ltd. 2012