EGFR protein overexpression correlates with chromosome 7 polysomy and poor prognostic parameters in clear cell renal cell carcinoma

Journal of Biomedical Science

Research

Gordana Đorđević¹  Nives Jonjić¹  Ita Hadžisejdić¹  Blaženka Grahovac¹  Koviljka Matušan Ilijaš¹  Anton Maričić² 

[1] Department of Pathology, School of Medicine, University of Rijeka, B. Branchetta 20, 51000, Rijeka, Croatia;Department of Urology, Rijeka University Hospital Centre, Krešimirova 42, 51000, Rijeka, Croatia;

关键词: Carcinoma;    Renal cell;    Chromosome 7;    EGFR;    In Situ Hybridization;    Fluorescence;    Prognosis;   

DOI  :  10.1186/1423-0127-19-40

 received in 2011-06-10, accepted in 2012-04-05,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe role of epidermal growth factor (EGF) and its receptor (EGFR) in the pathogenesis and progression of various malignant tumors has long been known, but there is still disagreement concerning prognostic significance of EGFR expression in clear cell renal cell carcinoma (CCRCC). The present study was designed to analyze more objectively the protein EGFR expression in CCRCC and to compare its value with EGFR gene copy number changes and clinicopathologic characteristics including patient survival.MethodsThe protein EGFR expression was analyzed immunohistochemically on 94 CCRCC, and gene copy number alterations of EGFR by FISH analysis on 41 CCRCC selected according to distinct membrane EGFR staining.ResultsMembrane EGFR expression in tumor cells was heterogeneous with respect to the proportion of positive cells and staining intensity. FISH analysis did not reveal EGFR gene amplification, while polysomy of chromosome 7 found in 41% was associated with higher EGFR membrane expression. Moreover, EGFR overexpression was associated with a higher nuclear grade, larger tumor size and shorter patient's survival, while there was no connection with pathological stage.ConclusionIn conclusion, the protein expression of EGFR had an impact on prognosis in patients with CCRCC, while an increased copy number of chromosome 7 could be the possible reason for EGFR protein overexpression in the absence of gene amplification.

【 授权许可】

Unknown   
© Đorđević et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.