Light microscopy applications in systems biology: opportunities and challenges

Cell Communication and Signaling

Review

Aleksandar Stojanovic¹  Karol Kozak²  Christophe Trefois³  Aidos Sagatovich Baumuratov³  Paul Paul Michel Aloyse Antony³ 

[1] Interdisciplinary Centre for Security, Reliability and Trust (SnT), University of Luxembourg, Luxembourg City, Luxembourg;Light Microscopy Centre (LMSC), Institute for Biochemistry, ETH Zurich, Zurich, Switzerland;Medical Faculty, Technical University Dresden, Dresden, Germany;Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Esch-sur-Alzette, Luxembourg;

关键词: Microscopy;    Systems biology;    Image analysis;    Segmentation;    Features;    Machine learning;   

DOI  :  10.1186/1478-811X-11-24

 received in 2013-02-26, accepted in 2013-03-28,  发布年份 2013

来源: Springer

【 摘 要 】

Biological systems present multiple scales of complexity, ranging from molecules to entire populations. Light microscopy is one of the least invasive techniques used to access information from various biological scales in living cells. The combination of molecular biology and imaging provides a bottom-up tool for direct insight into how molecular processes work on a cellular scale. However, imaging can also be used as a top-down approach to study the behavior of a system without detailed prior knowledge about its underlying molecular mechanisms. In this review, we highlight the recent developments on microscopy-based systems analyses and discuss the complementary opportunities and different challenges with high-content screening and high-throughput imaging. Furthermore, we provide a comprehensive overview of the available platforms that can be used for image analysis, which enable community-driven efforts in the development of image-based systems biology.

【 授权许可】

CC BY   
© Antony et al.; licensee BioMed Central Ltd. 2013